碳纳米管／羟基磷灰石／壳聚糖骨修复材料的制备及细胞相容性研究  

The research about preparation process and cell compatibility of MWCNT/n-HA/CS bone repair materials

作　　者：钱炜[1] 喻爱喜[2] 漆白文[2] 张韬[2] 

机构地区：[1]湖北省十堰市人民医院骨关节科,442000 [2]武汉大学中南医院显微外科

出　　处：《中华显微外科杂志》2011年第4期301-304,358,共5页Chinese Journal of Microsurgery

基　　金：基金项目：湖北省科技厅攻关项目（2006AA412C37）

摘　　要：目的设计一种多壁碳纳米管／纳米羟基磷灰石／壳聚糖（MWCNT／n—HA／CS）复合材料的骨组织工程支架，并对这种支架进行理化性质检测及细胞相容性评价。方法采用溶液共混、冷冻干燥法制备MWCNT／n—HA／CS支架。通过扫描电镜、X线衍射和傅立叶红外光谱分析其微观形貌和组成：采用陶瓷试验系统进行支架的机械性能检测，并评价MWCNT的加入对支架的影响。使用第3代兔骨髓间充质干细胞（BMSCs），使用扫描电镜检测支架对其粘附作用，采用MTT法检测细胞在支架上增殖．并评价碳纳米管的加入对支架的细胞相容性影响。评估设计的支架是否可用于骨组织工程。结果MWCNT／n—HA／CS支架具有明确的多孔结构，孔隙率高（87．26％），碳纳米管的加入可明显提高断裂强度（提高66％），而对孔隙率无明显影响（下降3％），对HA、CS的理化性质无明显影响。BMSCs在支架上粘附、增殖正常，且MWCNT具有促进细胞增殖能力。结论MWCNT／n—HA／CS支架具有较好的机械性能、合适的孔径大小、良好的细胞相容性，有望用于骨组织工程。Objective To evaluate the pbysicoehemica] properties and bicompatibi]ity of carbon-nan- otubes/hydroxypatite/chitosan scafflod for bone tissue engineering. Methods MWCNT/n-HA/CS scaffolds wre generated by solution blending and freeze-drying technology. The morphology and composition of the scaffolds were analyzed by scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy, after this, the results of which mixed CNTS in scaffolds were evaluated. The effects of MWC- NT/n-HA/CS scaffolds on adherence and proliferation of rabbit bone marrow stroma cells were assessed by scaffolds surface seeding methods, and using scanning electron microscopy, MTT assay to observe their adhe- sion and proliferation on scaffolds. Results MWCNT/n-HA/CS scaffolds showed abundant homogeneous pores with （87.26%） porosity. 66% fracture strength of the scafflod was improved by MWCNT, and porosity decreased by 3%. Conclusion MWCNT/n-HA/CS scaffold can be prepared with solution blending and freeze drying process, which has fair poriness, good mechanical strength and tissue compatibility and can be applied as a bone graft material.

关 键 词：碳纳米管 羟基磷灰石 壳聚糖 组织工程 骨髓间充质干细胞 

分 类 号：R318.08[医药卫生—生物医学工程]