高效液相色谱法测定止喘合剂中黄芩苷的含量  

Determination of baicalin content in Zhichuan mixture by HPLC

作　　者：林晓珊[1] 李国成[1] 刘春霞[1] 汪锦飘[1] 

机构地区：[1]中山大学孙逸仙纪念医院药学部,广州510120

出　　处：《第三军医大学学报》2011年第16期1740-1742,共3页Journal of Third Military Medical University

摘　　要：目的建立止喘合剂中黄芩苷含量的测定方法。方法色谱柱为Diamonsil C18（250 mm×4.6 mm,5μm）,流动相为甲醇-5.0%磷酸（60∶40）,流速为1.0 ml/min,检测波长为280 nm,柱温为25℃。结果黄芩苷的线性范围为0.952~6.664μg/ml,回归方程为y=48.13647x-9.74570（r=0.999 98）,平均回收率为96.21%,相对标准偏差（RSD）为0.373 7%（n=6）。结论所建立方法简单、专属性强、重现性好,可用于止喘合剂中黄芩苷的测定。Objective To establish an accurate method for determining the baicalin content of Zhichuan mixture.Methods HPLC was performed using a Diamonsil C18 column（250 mm×4.6 mm,5 μm） and a mobile phase consisting of methanol and 0.5% phosphoric acid（60∶40） at a column temperature of 25 ℃,a mobile phase flow speed of 1.0 ml/min,and a UV detection wavelength of 280 nm.Results Baicalin showed a good linear relationship in the concentration range of 0.952 to 6.664 μg/ml,with a linear regression equation of y=48.13647x-9.74570（r=0.999 98）.The average recovery rate was 96.21%.The relative standard deviation（RSD） was 0.3737%（n=6）.Conclusion Above HPLC method is easy to perform and has high specificity and high repeatability.This method can be used in the determination of baicalin in Zhichuan mixture.

关 键 词：止喘合剂 黄芩苷 高效液相法 

分 类 号：R282.71[医药卫生—中药学] R289.5[医药卫生—中医学]