Gardiquimod增强人γδT细胞杀伤肿瘤细胞的研究  

Gardiquimod enhance tumor cell lysis of human γδT cells

作　　者：周燏[1] 刘军权[1] 周忠海[1] 吕小婷[1] 陈玲[1] 孙蕾清[1] 李昳[2] 陈复兴[1] 

机构地区：[1]中国人民解放军第九七医院肿瘤生物治疗中心,徐州221004 [2]中国人民解放军第九七医院检验科,徐州221004

出　　处：《免疫学杂志》2011年第8期653-656,共4页Immunological Journal

基　　金：2009年度军区医学科技创新课题项目(09MA037)

摘　　要：目的研究Toll样受体7激动剂（Gardiquimod）对人γδT细胞杀伤肿瘤作用的影响。方法以异戊烯焦磷酸法扩增人外周血γδT细胞。用不同浓度的Gardiquimod处理γδT细胞和肺癌细胞A549,MTT法检测细胞增殖情况。LDH法检测Gardiquimod处理后γδT细胞对A549的杀伤活性;流式细胞术检测处理前后γδT细胞上CD107a、穿孔素和颗粒酶的表达情况。结果 Gardiquimod在5.0~0.31μg/ml浓度可明显促进γδT细胞增殖,同时抑制A549增殖。Gardiquimod处理γδT细胞后,杀伤A549能力明显增强,且CD107a、颗粒酶及穿孔素的表达显著升高,与对照组相比,有统计学差异（P〈0.05）。结论 Gardiquimod通过增高γδT细胞上的CD107a、颗粒酶及穿孔素的表达来增强其杀伤肿瘤作用。The aim of this study was to investigate the anti-tumor effect of γδT cells after treaded by Toll-like receptor 7 agonist（Gardiquimod）.Human γδT cells were amplified by isopentenyl pyrophosphate from peripheral blood cells.The proliferation capacity of γδT cells and lung cancer cell A549 were measured with MTT assay after treated with different concentrations of Gardiquimod.Cytoxicity of γδT cells was detected with LDH assay,and the expression of granzyme,prforin and CD107a on γδT cells were measured by flow cytometry before and after the treatment.The results showed that Gardiquimod could significantly stimulate the proliferation of γδT cells and inhibit A549 cell under the concentration of 5.0 μg/ml.Cytoxicity and expression of granzyme,perforin and CD107a of γδT cells were increased after the treatment,and Gardiquimod could enhance anti-tumor effect of human γδT cells.

关 键 词：Gardiquimod ΓΔT细胞 肺癌细胞A549 杀伤肿瘤 

分 类 号：R392.11[医药卫生—免疫学]