RIG-I激活抑制肠道病毒71型的复制  

RIG-I activation inhibits enterovirus 71 replication

作　　者：杨彬[1] 季芬[1] 王凤龙[1] 

机构地区：[1]内蒙古农业大学兽医学院

出　　处：《免疫学杂志》2011年第8期679-682,共4页Immunological Journal

摘　　要：目的探讨细胞内病毒识别受体RIG-I激活后对EV71复制的影响。方法分别用Poly(I:C)和表达RIG-I N端的质粒转染RD细胞,24 h后感染EV71,感染24 h后收获细胞和上清。提取细胞RNA并反转录为cDNA,Real-time PCR测定细胞中RIG-I与IFN-β的mRNA的表达水平和EV71的RNA水平。用免疫印迹的方法检测RIG-I和EV71的蛋白表达水平。将感染病毒的细胞上清进行病毒滴度的测定。结果 RIG-I被激活之后RIG-I与IFN-β的mRNA的表达水平上升,并且RIG-I的蛋白表达可以被检测到。感染细胞中的EV71的RNA水平和病毒蛋白表达水平下降,并且病毒滴度显著降低。结论 RIG-I的激活以及其诱导的IFN-β对EV71的复制有抑制效应。Activation of innate immune pathogen sensor retinoic acid-inducible gene I （RIG-I） has recently been shown to induce antiviral state. In this study, we aim to study the effect of the intracellular viral sensor RIG-I activation on EV71. RD cells were transfected with Poly （I：C） or plasmid expressing RIG-I N-terminal. At 24 h after transfection, cells were infected with EV71; at 24 h post-infection, cells were harvested for RNA and protein analysis and cell culture supernatants were collected for determination of viral titer. The transcription of IFN-β and RIG-I was detected by Real-Time PCR; the protein expression of RIG-I and EV71 was detected by immunoblotting. The results showed that EV71 replication was inhibited after RIG-I activation. These findings in-dicate that RIG-I activation and subsequent induction of IFN-β have inhibitory effects on EV71 replication.

关 键 词：维甲酸诱导基因I 肠道病毒71型 干扰素-Β 

分 类 号：R392.12[医药卫生—免疫学]