缬沙坦对急性肺损伤大鼠TGF-β／Smads信号通路的影响  

Effects of valsartan on transforming growth factor-β1 , Smad in an acute lung injury rat model

作　　者：樊麦英[1] 肖奇明[2] 

机构地区：[1]湖南省人民医院急诊科,长沙410005 [2]中南大学湘雅医院呼吸科

出　　处：《中国医师杂志》2011年第7期909-911,916,共4页Journal of Chinese Physician

摘　　要：目的观察缬沙坦对急性肺损伤大鼠TGF-β／Smad信号通路的影响。方法24只SD雄性大鼠随机分为对照组、模型组和缬沙坦组三组，每组8只。缬沙坦组于气管内滴注博莱霉素（BLM）生理盐水溶液（5mg／kg）以复制急性肺损伤动物模型，并于造模当天每日给予缬沙坦（20mg／kg）灌胃；模型组以生理盐水代替缬沙坦灌胃；对照组则均用生理盐水代替BLM和缬沙坦。各组动物均于制模开始后第7天处死，分取肺组织行病理切片HE染色观察肺损伤程度、免疫组化技术检测肺组织TGF—β1、Smad2／3和Smad7蛋白的表达水平。结果缬沙坦组大鼠肺组织损伤程度较模型组比较明显减少（P〈0．01）。模型组肺组织内TGF-β1、Smad2／3蛋白表达水平较对照组明显增强（P〈0．01）。缬沙坦组TGF-β1、Smad2／3蛋白表达水平较模型组降低（P〈0．01）。Smad7蛋白在模型组肺组织内表达明显低于对照组（0．23±0．02vs0．36±0．03，P〈0．01），缬沙坦组Smad7蛋白表达同模型组比较明显增强（P〈0．01）。结论缬沙坦可下调急性肺损伤大鼠肺组织内TGF—β、Smad2／3蛋白表达，上调Smad7蛋白表达，从而阻断TGF-β／Smad信号通路，减轻急性肺损伤程度。Objective To observe the effect of Valsartan on a rat model of acute lung injury and the expression of transforming growth factor-β,TGF-β） ,Smad2/3, Smad7. Methods Twenty-four male adult Sprague-Dawley rats were random divided into three groups ： The bleomycin （ BLM ） group, the control group, and the Valsartan group. Each group contained eight rats. The Valsartan group was treated with Valsartan everyday at a dose of 20 mg/kg after a single intratracheal instillation of bleomyein at a dose of 5mg/ kg. BLM group was treated with saline instead of Valsartan after an instillation of bleomycin. The control group was treated with saline instead of Valsartan and bleomyein. Each group was killed at the 7th day after instillation. The lung tissues were harvested for H.E. stain, the immunohistochemistry was used to detect the expressions of TGF-β1 , Smad2/3, and Smad7. Results The degree of alveolitis in the Valsartan group was ameliorated, compared with those in BLM group （ P 〈 0. 01 ）. The expressions of TGF-β1 and Smad2/ 3 in lung tissue of the Valsartan group were significantly lower than that of BLM group（ P 〈 0. 01 ）. The expressions of Smad7 in lung tissue of the Valsartan group were significantly higher than that of BLM group （0. 23±0. 02 vs 0. 36 ± 0. 03, P 〈 0. 01 ）. Conclusions Valsartan could alleviate acute lung injury in rats, which probably be due to the expression decrease of TGF-β1 and Smad2/3 and the expression increase of Smad7 in lung tissues.

关 键 词：缬氨酸/类似物和衍生物/药理学 呼吸窘迫综合征 成人/代谢 转化生长因子β/生物合成 Smad蛋白质类/生物合成 肺/损伤/代谢 

分 类 号：R965[医药卫生—药理学]