补充谷氨酰胺对过度训练大鼠腹膜巨噬细胞IGF-1和MGF基因表达的影响  

Supplementing Glutamine Affects the IGF-1 and MGF mRNA Levels in Peritoneal Macrophages of Overtrained Rats

作　　者：肖卫华[1,2] 陈佩杰[1] 

机构地区：[1]上海体育学院运动科学学院,上海200438 [2]湘南学院体育系

出　　处：《中国运动医学杂志》2011年第7期634-638,共5页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金资助项目(30971422);上海市重点学科建设资助项目(S30802);上海体育学院研究生创新基金(yjscx201004)

摘　　要：目的:了解补充谷氨酰胺对过度训练大鼠腹膜巨噬细胞胰岛素样生长因子1(IGF-1)和机械生长因子(MGF)基因表达的影响。方法:8周龄健康雄性Wistar大鼠40只,随机分为安静对照组(C)、过度训练组(E)、过度训练补充谷氨酰胺组(EG)。后两组根据取材时间不同分为2组:运动后36 h取材组(E1、EG1),运动后7天取材组(E2、EG2)。总计5组,每组8只,除C组外,其他4组进行11周递增负荷跑台训练。EG组从第5周开始至第8周灌胃补充谷氨酰胺(0.8 g/kg/d),以后几周加至饮用水补充,剂量逐周加大到1.1 g/kg/d。断头处死大鼠并分离纯化腹膜巨噬细胞,采用荧光定量PCR技术测定IGF-1和MGF基因表达。结果:安静状态下巨噬细胞即可表达IGF-1和MGF。11周过度训练后36 h,巨噬细胞IGF-1、MGF表达量显著增加,分别约为安静对照组的21倍和92倍(P<0.01)。EG1组IGF-1、MGF表达显著增加,分别约为安静对照组的10倍和37倍(P<0.01),但显著低于E1组(P<0.01)。停训后恢复7天,E2组、EG2组IGF-1、MGF表达量分别与E1组、EG1组相比均显著下降(P<0.01),但与C组相比差异无统计学意义。结论:静息态巨噬细胞可表达IGF-1和MGF;过度训练可增强巨噬细胞IGF-1和MGF表达,MGF表达对运动应激更敏感;补充谷氨酰胺可部分抑制巨噬细胞IGF-1和MGF对过度训练的应答。Objective To investigate the effect of supplementation of glutamine（Gln）on the IGF-1 and MGF mRNA levels in peritoneal macrophages of rats with overtraining.Methods Forty male Wistar rats were randomly divided into following groups：sedentary group（C,n = 8）,overtraining group（E）,overtraining and supplementation of Gln group（EG）.The groups E and EG were respectively subdivided into two groups which were sacrificed 36 hours（E1,EG1,n = 8）and 7 days（E2,EG2,n = 8）after the last training.All groups except the C underwent standard treadmill training with an increasing load for 11 weeks.And group EG was supplied with Gln（0.8 g/kg/d）orally from the fifth week to eighth week,and the Gln was added to drinking water from the eighth week to eleventh week（dose increased by week,finally to 1.1 g/kg/d）.Peritoneal macrophages were isolated and purified after all rats were sacrificed by decapitation.Real time-PCR was used to detect the mRNA expression of IGF-1 and mechano growth factor（MGF）.Results The expression of IGF-1 and MGF in peritoneal macrophages could be detected at rest.The expression of IGF-1 and MGF in group E1 significantly increased by 21 times and 92 times respectively（P〈0.01）when compared with the group C.The IGF-1 and MGF mRNA levels in group EG1 significantly increased by 10 times and 37 times respectively（P〈0.01）as compared with the group C,however,they were significant lower than in group E1（P〈0.01）.In the recovery period;the IGF-1 and MGF gene expression in groups E2 and EG2 were declined significantly when compared with that in groups E1 and EG1（P〈0.01）,however,there were no significant differences when compared with the group C（P〈0.05）.Conclusion The expression of IGF-1 and MGF in peritoneal macrophages could be detected at rest.Overtraining could significantly enhance the expression of IGF-1 and MGF in macrophages.And the MGF mRNA level was more sensitive to the exercise stress than IGF-1.Supplementing Glutamine could partially inhibit the

关 键 词：巨噬细胞 过度训练 谷氨酰胺 胰岛素样生长因子1 机械生长因子 

分 类 号：R87[医药卫生—运动医学]