D001阳离子交换树脂分离红曲霉发酵液中γ-氨基丁酸的研究  

Study on Separation of γ-Aminobutyric Acid from Monascus Fermentation Broth by D001 Ion-exchange Resins

作　　者：嵇豪[1] 蒋冬花[1] 周琴[1] 汪鹏荣[1] 董夏梦[1] 蔡琪敏[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,金华321004

出　　处：《中国粮油学报》2011年第8期95-99,共5页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31070008);浙江省自然科学基金(Y3090343)

摘　　要：研究了红曲霉发酵液中的γ-氨基丁酸(GABA)分离纯化工艺。对3种脱色剂进行筛选,D101树脂对红曲发酵液具有最高的脱色率和GABA得率。分别以静态和动态吸附-解析方法考察D001离子交换树脂对GABA的最优分离条件,结果表明:脱色发酵液pH下上样,上样流速为1 BV/h,采用水和2 mol/L氨水两步洗脱,洗脱液浓缩后结晶为透明针状,GABA的总得率为45.4%。The separation process of T -Aminobutyric acid （GABA） from monascus fermentation broth was researched. Three types of decolorants were selected, and the D101 exhibited high decoloring ratio and high GABA yield. The static and dynamic adsorption - desorption performances were investigated to obtain optimized conditions of GABA separation by D001 ion -exchange resins. The result showed that the optimum parameters were as follows ：original pH of decolor fermentation broth, sample flow - rate of 1 BV/h, elution method of two steps by using water and 2 mol/L aqueous ammonia. The transparence needle crystal of GABA was obtained after the effluent concentrated. The total recovery rate was 45.4%.

关 键 词：Γ-氨基丁酸 红曲霉发酵液 D001阳离子树脂 分离 

分 类 号：Q819[生物学—生物工程]