DA201-C大孔吸附树脂对腐乳多肽脱盐作用的研究  

Study on Desalination of DA201-C Macroporous Adsorption Resin on Fermented Bean Curd Polypeptide

作　　者：范文[1] 阮长青[1] 王鹤霖[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319

出　　处：《中国粮油学报》2011年第8期105-108,共4页Journal of the Chinese Cereals and Oils Association

摘　　要：为去除腐乳中的盐分,利于其中活性肽的分离纯化,以腐乳多肽的回收率为指标,采用DA201-C大孔吸附树脂对超滤的水溶性低聚肽的脱盐工艺进行了研究。结果表明:DA201-C大孔吸附树脂对腐乳多肽较佳的脱盐工艺条件为上样浓度45 mg/mL、洗脱流速120 mL/h、解吸剂为70%乙醇。腐乳多肽经DA201-C大孔吸附树脂处理后脱盐率达到98.19%,肽回收率大于90%,抗氧化活性得到提高。利用DA201-C大孔吸附树脂是进行腐乳多肽脱盐处理的一种简便有效方法。In order to remove the salt of fermented bean curd, benefit the separation and purification of active peptide and realize the target of the recovery rate of fermented bean curd polypeptides, the desalting technology of fermented bean curd polypeptides which were water - soluble after ultra_filtration by DA201 - C maeroporous adsorption resin was investigated. The results indicated that the optimum condition for desalting was obtained as follows：loading sample concentration of 45 mg/mL,flow speed of 120 mL/h and 70% alcohol as eluent. Under these conditions, the ratio of fermented bean curd polypeptides desalination reached to 98.19%, and the recovery of peptides was more than 90%. Moreover, its antioxidant activity was enhanced. In conclusion ,the desalination method by using DA201 - C macroporous adsorption resin applied to fermented bean curd polypeptides was a simple and available method.

关 键 词：大孔吸附树脂 腐乳多肽 脱盐 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]