胆木愈伤组织与悬浮细胞培养研究  

Callus Induction and Cell Suspension Culture of Nauclea officinalis

作　　者：王和飞[1] 梁柳[1] 张燕[1] 刘进平[2] 

机构地区：[1]琼州学院生物科学与技术学院,海南五指山572200 [2]海南大学农学院,海南儋州571737

出　　处：《热带农业科学》2011年第6期53-57,共5页Chinese Journal of Tropical Agriculture

基　　金：海南省教育厅高校科研资助项目"胆木微繁体系建立的研究"(No.Hjkj2010-44)

摘　　要：通过组织培养技术,利用胆木(Nauclea officinalis)种子无菌实生苗的茎段、叶片、叶柄等进行愈伤组织的诱导及悬浮细胞的培养。结果如下:(1)以WPM为基本培养基,获得愈伤组织高诱导率的激素种类及浓度为①0.5 mg/L 2,4-D、②0.5～1.5 mg/L IBA+(0.0,0.5 mg/L)6-BA,诱导率达到100%;(2)在外植体的差异上,茎段和叶片出现愈伤组织的时间较早、增殖系数最大,可达15倍;(3)在培养基1/2WPM+0.5 mg/L2,4-D+30 g/L蔗糖中,继代周期为14～17 d,胆木细胞悬浮培养增殖率可达30.16%。Callus induction and suspension culture of Nauclea officinalis were conducted using stem segments,leaves,petioles,etc.of in vitro seedlings as explants.The results showed that the best combinations of plant growth regulators for callus induction on WPM were ①0.5 mg/L 2,4-D,and ②0.5-1.5 mg/L IBA＋（0.0,0.5 mg/L） 6-BA,with frequencies of callus induction being 100%.The better explants were stem segments and leaves since calli appeared earlier on them and their multiplication coefficients were up to 15.The proliferation rate of cell suspension culture of Nauclea officinalis on 1/2WPM＋0.5 mg/L 2,4-D＋30 g/L sugar was up to 30.16 for a subculture period of about 14-17 days.

关 键 词：胆木 愈伤组织 悬浮培养 增殖系数 

分 类 号：Q946[生物学—植物学]