宫颈脱落细胞高危人乳头瘤病毒DNA分型定量的临床应用  

Clinical application of high risk-human papillom virus-DNA typing and quantification in exfoliated cervical cells

作　　者：杨秀[1] 童红莉[1] 朱智慧[1] 邓心新[1] 田亚平[1] 温新宇[1] 

机构地区：[1]解放军总医院生化科,北京100853

出　　处：《军医进修学院学报》2011年第8期820-821,824,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家科技支撑计划项目(2009BAI86B05)~~

摘　　要：目的探讨宫颈脱落细胞高危人乳头瘤病毒DNA(HR-HPV-DNA)分型定量在宫颈癌防治中的意义。方法采用实时定量PCR-双色荧光探针法,对1 417例体检妇女进行宫颈脱落细胞作HR-HPV-DNA分型定量检测和TCT检测。结果 HR-HPV16是HR-HPV单型别中阳性率最高的型别(19.14%)。HR-HPV-DNA总体阳性率为13.76%,HR-HPV-DNA阳性率和水平随着细胞学诊断的严重程度增加而升高。在宫颈上皮内瘤变(CIN)组,HR-HPV-DNA水平高于正常组、萎缩性鳞状上皮细胞组、轻度炎症组和中度炎症组(P<0.05)。非典型鳞状上皮细胞组HR-HPV-DNA水平高于轻度炎症组(P<0.05)。结论细胞学诊断的严重程度不仅与HR-HPV的型别有关,还与HR-HPV-DNA的水平有关。Objective To study the significance of high risk-human papillom virus-DNA（HR-HPV-DNA） typing and quantification in prevention and treatment of cervical cancer.Methods HR-HPV-DNA was typed and quantified was detected in exfoliated cervical cells from 1 417 normal females by real-time dual fluorescent probes PCR and 1 417 cases were detected using TCT.Results The positive rate of HR-HPV16 was the highest in all types of HR-HPV,accounting for 19.14%.The total positive rate of HR-HPV-DNA was 13.76%,which increased with the increasing severity of cervical cancer.The HR-HPV-DNA level was significantly higher in cervical intraepithelial neoplasia（CIN） group and atypical squamous cell group than in normal group,atrophic squamous cell group,mild and moderate inflammation groups（P0.05）.Conclusion The severity of cervical cancer is related not only with the HR-HPV type but also with the HR-HPV-DNA level.

关 键 词：高危人乳头瘤病毒 宫颈癌 宫颈脱落细胞 DNA分型 

分 类 号：R373[医药卫生—病原生物学]