玉米自交系游离氨基酸HPLC测定与分析  

Detection and analysis of free amino acids in main maize inbred lines by HPLC method

作　　者：姬玉梅 赵丽维[1] 

机构地区：[1]河南科技学院河南省高等学校作物分子育种重点学科开放实验室,河南新乡453003 [2]鹤壁职业技术学院,河南鹤壁458030

出　　处：《浙江农业学报》2011年第4期651-654,共4页Acta Agriculturae Zhejiangensis

基　　金：河南省自然科学基金资助项目(0511031100)

摘　　要：为了解玉米自交系的氨基酸品质性状,筛选出高游离氨基酸含量的自交系,为玉米育种和品质改良提供优质种质资源。试验利用HPLC方法,对高赖氨酸玉米‘Opaque-2’和13个常用玉米自交系的16种游离氨基酸含量进行了检测。结果表明,‘Opaque-2’材料中检测出15种游离氨基酸,总游离氨基酸含量达干物质总量的3.42%,赖氨酸含量达0.28%。13个常用自交系除1个含量稍高外,其余12个自交系游离氨基酸和必需氨基酸含量都很低,赖氨酸含量仅是‘Opaque-2’的0～34%。该结果说明目前常用玉米主要品种亲本自交系的必需氨基酸营养品质基因是低水平类型。In order to study the quality and traits of amino acid in maize inbred lines,and to screen out the inbred line with high levels of free amino acids to provide high-quality germplasm resources for maize breeding and quality improvement,sixteen kinds of free amino acids extracted form high-lysine corn Opaque-2 and thirteen commonly used inbred lines were detected using HPLC method.The result showed that fifteen kinds of amino acids were detected in Opaque-2,and the content of all free amino acids accounted up to 3.42 percent of total dry matter,while that of lysine was up to 0.28 percent.The content of free amino acids and essential amino acids were very low in the thirteen commonly used inbred lines with the exception of only one line,while the content of lysine was only 0-34 percent of the content of lysine in Opaque-2.In summary,it showed that the genes of essential amino acids in their parents inbred lines of commonly used maize varieties with excellent nutritional quality were scarce.

关 键 词：玉米 自交系 游离赖氨酸 种质资源 高效液相色谱 

分 类 号：S513[农业科学—作物学]