实时荧光定量PCR在植物病害流行学中的应用  

Application of real-time quantitative PCR to epidemiology of plant diseases

作　　者：郑亚明[1] 骆勇[2] 周益林[1] 段霞瑜[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]中国农业大学农学与生物技术学院,北京100193

出　　处：《植物保护》2011年第4期18-22,共5页Plant Protection

基　　金：公益性行业科研专项(3-15);国家重点基础研究发展计划(2010CB951503);国家科技支撑计划项目(2006BAD08A05)

摘　　要：实时荧光定量PCR广泛应用于植物病理学研究的各个领域。近年来已开始应用于植物病害流行学的定量研究中,本文对近年来实时荧光定量PCR技术在病菌初始菌源的定量分析、病害流行动态的监测、寄主抗病性的快速鉴定和植物病原菌的抗药性监测等方面的应用进行了综述。Real time quantitative PCR has been widely used in various areas of plant pathology research. In recent years, it has been applied to the quantitative studies of epidemiology of plant diseases. The applications of real time PCR to epidemiology of plant diseases were reviewed, including the quantification of initial inoculum of pathogens, the monitoring of disease epidemics, the identification of host resistance, monitoring of fungicide resistance of the pathogen and so on.

关 键 词：实时荧光定量PCR 植物病害流行学 应用 

分 类 号：S432.1[农业科学—植物病理学]