人胰腺癌裸鼠异种移植瘤模型的生物发光成像和超声成像比较  

Comparison of Bioluminescent Imaging and Ultrasound Imaging in a Mouse Model of Pancreatic Cancer

作　　者：李小颖[1] 董伟[1] 张连峰[1] 

机构地区：[1]中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国比较医学杂志》2011年第7期13-16,F0002,共5页Chinese Journal of Comparative Medicine

基　　金：卫生部项目,实验动物和人类疾病动物模型资源扩展(200802036);十一五新药专项支持(2009ZX09501-026)

摘　　要：目的利用荧光素酶基因标记的人胰腺癌细胞株Capan-2建立胰腺癌裸鼠移植模型,评价生物发光和小动物超声成像在移植瘤模型建立中的作用。方法将表达荧光素酶基因的真核表达载体转入人胰腺癌细胞Capan-2,将1×106人胰腺癌细胞悬液分别接种于裸鼠胰腺和右后肢皮下,使其成瘤。生物发光成像和小动物超声成像系统观察肿瘤的生长情况。结果肿瘤细胞原位移植成功率为75%,皮下移植成功率为100%。生物发光成像系统在肿瘤细胞原位接种第7天,可以观察到肿瘤发光;小动物超声成像系统在肿瘤细胞皮下接种第7天,可以测量肿瘤的大小,但在肿瘤细胞原位接种的第7天不能测量肿瘤的大小。另外肿瘤细胞在裸鼠皮下生长的速度比原位生长速度快3倍左右。结论生物发光成像系统更适用于肿瘤早期监测,为深入研究胰腺癌的发生发展、侵袭转移机制提供理想工具。Objective To establishment of human pancreatic cancer mouse model labeled with luciferase and evaluate bioluminescent imaging and ultrasound imaging for tumor burden and tumor progression in a mouse model of pancreatic cancer.Method The human pancreatic cancer cell line Capan-2,engineered for stable,high-level expression of luciferin（LUC）,was implanted into pancreas and subcutaneous tissue of nude mice.The tumors were allowed to grow over a period of one to several weeks during which time the mice were imaged using both bioluminescent imaging and ultrasound imaging to monitor tumor growth.Result The successful rate of orthotopic and subcutaneous transplantation was 75% and 100% separately.The whole-body optical imaging found that the fluorescence could be detected after 7 days of orthotopic transplantation.The size of tumor can be measured after 7 days of subcutaneous transplantation by ultrasound imaging but it can＇t be done in orthotopic transplantation.Otherwise the growth rate of tumor besides subcutaneous is about 3 times faster than pancreas.Conclusion Bioluminescent imaging is more suitable for monitoring the tumor at the early stage,and provides an ideal tool to further study the development of tumor and the mechanism of metastasis.

关 键 词：人胰腺癌细胞 生物发光 小动物超声 原位移植 皮下移植 

分 类 号：R332[医药卫生—人体生理学]