野生大豆GsbZIP33基因的分离及胁迫耐性分析  

Isolation and Tolerance Analysis of GsbZIP33 Gene Linked to Response on Stress in Glycine soja

作　　者：才华[1] 朱延明[1] 柏锡[1] 纪巍[1] 李勇[1] 王冬冬[1] 孙晓丽[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《分子植物育种》2011年第4期397-401,共5页Molecular Plant Breeding

基　　金：国家转基因生物新品种培育重大专项(2008ZX08004);国家自然科学基金资助项目(30471059);黑龙江省教育厅科技项目(11521021);黑龙江省博士后基金(LBH-Z08253)项目共同资助

摘　　要：bZIP(basic leucine zipper)类转录因子在植物的生长发育、光形态建成、光信号传导及非生物胁迫反应中发挥重要的作用,已成为非生物胁迫基因工程领域的研究热点。本研究以野生大豆(Glycine soja)为材料,利用同源克隆的方法克隆了GsbZIP33转录因子基因,该基因与大豆GmbZIP33(DQ787064)及拟南芥AtbZIP53(NP_171677.1)分别具有95%和54%的相似性,同属于bZIP-S亚家族的成员。GsbZIP33能够响应高盐、干旱和低温胁迫,并且在根和叶中具有不同的表达模式。超量表达GsbZIP33基因的拟南芥对盐胁迫的敏感性提高。以上结果表明,GsbZIP33基因是野生大豆bZIP家族的新成员,并且参与植物非生物胁迫反应过程,意味着该基因在非生物胁迫基因工程研究领域具有良好的理论研究和实际应用价值。bZIP（basic leucine zipper） transcription factors play important roles in various biological processes of plant growth and development,photomorphogenesis,light signal transduction and abiotic stress response.It has become a new research focus in the abiotic-stress field.Based on that,a new bZIP gene was cloned from Glycine soja by homologous cloning mathod,and has 95% and 54% similarity with bZIP33 of Glycine max（DQ787064） and AtbZIP53（NP_171677.1）,respectivily.Semi-quantitative RT-PCR showed the expression level of GsbZIP33 was induced by drought,low temperature and salt stresses,but there existed difference between leaf and root in G.soja.Arabidopsis thaliana plants overexpressing GsbZIP33 showed higher sensitivity under salt stress.All results showed that GsbZIP33 perhaps was a new member of bZIP family in G.soja,and is closely related to salt stress,so it can either be used as a new resource in gene engineering on stress tolerance or be further studied to provide more information for the researches on the mechanism of stress tolerance in plant.

关 键 词：野生大豆 转录因子 GsbZIP33 非生物胁迫 

分 类 号：S565.1[农业科学—作物学]