P物质增强失血性休克大鼠离体淋巴管的泵功能  

Substance P enhances pump function of isolated lymphatics from hemorrhagic shock rats

作　　者：秦立鹏[1] 牛春雨[1] 赵自刚[1] 张静[1] 张玉平[1] 

机构地区：[1]河北北方学院微循环研究所,基础医学院病理生理教研室,河北张家口075000

出　　处：《中国病理生理杂志》2011年第7期1323-1328,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30770845);河北省自然科学基金资助项目(No.C2008000503);河北省教育厅科学研究重点资助项目(No.ZH2007101)

摘　　要：目的:建立离体淋巴管灌流技术,观察P物质(SP)对失血性休克(HS)发展进程中淋巴管收缩性的影响。方法:Wistar雄性大鼠随机分为对照组(仅麻醉与手术)和HS组[经股动脉放血,维持平均动脉压(40±2)mmHg,分为休克0 h、0.5 h、1 h、2 h、3 h 5个亚组]。各组在相应时点分离胸导管,制备淋巴管条,在3 cmH2O跨壁压下离体灌流,分别给予从低到高浓度的SP,测量淋巴管收缩末期口径、舒张末期口径、收缩频率(CF)和被动管径,计算收缩幅度(CA)、泵流分数(FPF)和紧张指数(TI)。结果:SP对各组淋巴管的CF、TI和FPF均有提高作用,且随着SP浓度的增加,这种作用也逐渐增强;SP浓度自3×10-8mol/L起,将休克2 h和3 h淋巴管的CF、TI和FPF提高至(或超过)实验前对照组水平。同一浓度下,SP对各组淋巴管CA的影响无统计学差异;但随着SP浓度增高,各组淋巴管CA值出现了下降趋势。结论:SP不仅具有增强生理状态下淋巴管泵功能的作用,更重要的是可增强休克各期淋巴管的泵功能。AIM： To establish a technique of lymphatic perfusion in vitro and to determine the effect of substance P（SP） on lymphatic contractility during the process of hemorrhagic shock（HS）.METHODS： Male Wistar rats were randomly divided into control group（surgical procedure only） and HS group [the rats underwent bloodletting from femoral artery while the mean arterial pressure was maintained at（40±2） mmHg,and were further divided into 5 subgroups： shock 0 h,0.5 h,1 h,2 h and 3 h groups].Thoracic ducts were isolated from HS rats at the corresponding time points.The segment of thoracic duct was prepared,perfused in vitro at the transmural pressure of 3 cmH2O and stimulated with gradient concentrations of SP to measure the lymphatic contractile activity.The end-systolic diameter,end-diastolic diameter,contraction frequency（CF） and passive diameter of isolated lymphatics were measured.The contraction amplitude（CA）,tonic index（TI） and fractional pump flow（FPF） were also calculated.RESULTS： SP increased lymphatic CF,TI and FPF,and the effects were enhanced with the increase in the concentration of SP.The CF,TI and FPF of 2 h-and 3 h-shocked lymphatics were elevated to/over the value of baseline levels before the experiment by SP at the concentration starting from 3×10-8 mol/L.At the same concentration of SP,the CA of lymphatics showed no significant statistical difference among the groups.However,with the increase in SP concentration,the lymphatic CA had a downward trend in all groups.CONCLUSION： SP enhances the pump function of lymphatics not only under physiological condition,but also in shock during different stages.

关 键 词：休克 出血性 淋巴管 收缩性 P物质 

分 类 号：R331.4[医药卫生—人体生理学] R322.26[医药卫生—基础医学]