骨髓间充质干细胞治疗兔膝RA模型软骨损伤的MRI评价  

MRI evaluation of bone marrow mesenchymal stem cells in the treatment of cartilage damage in RA rabbit model

作　　者：周钟珩[1] 崔延安[1] 刘钊[1] 刘红霞[1] 黄海青[1] 张全彬[2] 

机构地区：[1]江苏省中医院MR室,江苏南京210029 [2]南京医科大学附属南京第一医院骨科中心,江苏南京210006

出　　处：《中国临床医学影像杂志》2011年第7期479-482,共4页Journal of China Clinic Medical Imaging

基　　金：江苏省自然科学基金面上项目(编号:BK2009460)

摘　　要：目的:用骨髓间充质干细胞治疗兔早期类风湿关节炎,运用MRI测量软骨厚度,观察前后改变,评价治疗效果。方法:选取42只新西兰大白兔,用卵蛋白造模,造模第4周(治疗前)取6只兔行MR和病理检查以对照,余36只兔随机分为RA模型组和BMSCs治疗组,每组18只,两组于治疗后1、2、3个月分别选取6只兔行MR和病理检查。对病变膝关节的软骨厚度进行MRI测量及病理学评分,并对数据进行统计学分析。结果:RA组经1、2、3个月后,软骨厚度变薄,病理学评分增加。BMSCs治疗组治疗1、2、3个月后,关节软骨厚度恢复,病理学评分减低。MRI测量数据和病理学评分有相关性。结论:MRI可用于评价BMSCs治疗早期类风湿关节炎关节软骨的改变。Objective：To observe the effects of bone marrow stem cells（BMSCs） transplantation in treating rheumatoid arthritis（RA） in rabbits by measuring the thickness of knee joint articular cartilage using MRI.Methods：Ovalbumin was used to induce right knee rheumatoid arthritis in 42 New Zealand white rabbits.Four wks later,MRI and pathologic examination were carried out in 6 rabbits,the remaining 36 rabbits were randomly and equally divided into the RA group as control and the BMSCs transplantation group.Normal saline was injected into the right knee joint of the former group and same volume of BMSCs was injected into the right knee joint of the latter group.At 1,2,3 months after injection,6 rabbits at each time point of both groups were given MRI and pathologic examination respectively,the thickness of knee articular cartilage was measured by MRI and the pathologic changes were observed and scorings were recorded.Results：After 1,2,3 months the cartilage in the RA group showed thinning and damaged with high pathologic score.While the cartilage was thickened with lower pathologic scorings in the BMSCs group.MRI measurements and pathology scoring correlated well.Conclusion：MRI can be used to evaluate the effect of BMSCs in the treatment of cartilage in early RA.

关 键 词：间质干细胞 关节炎 类风湿 兔 磁共振成像 

分 类 号：R593.22[医药卫生—内科学] R445.2[医药卫生—临床医学]