CGP37157对静息期心肌细胞钙循环的影响  

The effect of CGP37157 on diastolic myocardial calcium cycling

作　　者：王永亮[1] 沈亚峰[1] 刘关省[1] 胡淑婷[1] 汤莹[1] 杨勇骥[1] 

机构地区：[1]第二军医大学基础部生物物理学教研室,上海200433

出　　处：《电子显微学报》2011年第3期239-243,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.30170253);国家重点基础研究发展计划(973计划)资助项目(No.2006CB503807);军队医药卫生"十一五"科研项目自助(No.06MB202)

摘　　要：目的:研究离子通道阻断剂CGP37157对成年大鼠静息期心肌细胞钙循环的影响。方法:(1)32只雄性SD大鼠随机等分为4组,用于测定心肌组织中cAMP的含量。对照组不灌流CGP37157,实验组分3个时间梯度:用10μmol/L CGP37157灌流心脏15 min、30 min和60 min;(2)20只雄性SD大鼠随机均分为两组,对照组和实验组(细胞池中加入10μmol/L CGP37157)采用常规酶解法分离心肌细胞,激光扫描共聚焦显微镜记录钙瞬变;利用SPSS11.5统计软件进行数据处理,所有数据以均数±标准差(x珋±SD)表示,两组间均数的比较采用t检验。P<0.05为差异有统计学意义。结果:(1)心肌组织中cAMP含量的比较:CGP37157引起心肌组织cAMP的含量显著增加(n=8,P<0.05),对照组、15 min、30 min和60 min各组测得的cAMP的含量分别为(791±35)pmol/g、(1 101±55)pmol/g、(1 525±40)pmol/g、(2 017±60)pmol/g;60 min组较30 min组cAMP含量显著增加(n=8,P<0.05);(2)对静息期肌浆网钙循环的影响:实验组钙存储改变量(20.2±0.8)显著高于对照组(0.3±0.3;n=10,P<0.01);实验组钙释放速率变化(5.02±0.015)比对照组显著增大(1.01±0.006;n=10,P<0.01);实验组钙回摄速率变化(1.08±0.15)与对照组没有差异(0.99±0.09;n=10,P>0.05)。结论:CGP37157导致静息期心肌细胞钙循环的异常,原因可能为其导致PKA通路异常。Objective：Study the effect of CGP37157 on diastolic myocardial calcium cycling.Methods：（1） 32 male SD rats were randomly and equally divided into four groups： control group（without CGP37157）,experiment groups with 10 μmol/L CGP37157 for 15min,30min and 60min,respectively.cAMP content in cardiac tissue was measured;（2） 20 male SD rats were randomly and equally divided into two groups： control group（without CGP37157） and experiment group（with 10 μmol/L CGP37157）.Single cardiomyocytes of the rat heart were isolated by an enzymatic dissociation method and laser scanning confocal microscope was used to record intracellular Ca2＋ transients.SPSS11.5 software was used for statistical analysis.All the data were presented as mean±SD,and t-test was used for the statistical analysis.P-values of 0.05 was considered significant.Results：（1） cAMP content in experiment groups（1 101±55）pmol/g,（1 525±40）pmol/g,（2 017±60）pmol/g was increased compared with control group（791±35）pmol/g;（n=8,P0.05）,respectively;cAMP content of 60 min group was increased compared with that of 30min group（n=8,P0.05）;（2） Diastolic calcium cycling in cardiac myocytes： the change of calcium storage was increased in experiment group（20.2±0.8） compared with control group（0.3±0.3,n=10,P0.01）;the rate of calcium release was increased in experiment group（5.02±0.015） compared with control group（1.01±0.006,n=10,P0.01）;the rate of calcium uptake（1.08±0.15 vs 0.99±0.09,n=10,P0.05） failed to alter in experiment group compared with control group.Conclusion：The present results suggest that CGP37157 results in abnormal cardiac calcium cycling,and this is associated with abnormal PKA pathway.

关 键 词：CGP37157 心肌细胞 钙循环 

分 类 号：R541[医药卫生—心血管疾病]