实时荧光定量PCR在铁路运输动物携带甲型流感病毒检测中的应用  

Application of real-time fluorescence and quantitative PCR in detecting influenza A virus in animals transported by railway

作　　者：区伟全[1] 卢国钧 

机构地区：[1]广州市番禺区疾病预防控制中心,广东广州511400 [2]广州市铁路疾病预防控制中心,广东广州510010

出　　处：《热带医学杂志》2011年第7期828-829,共2页Journal of Tropical Medicine

摘　　要：目的探讨应用实时荧光PCR对铁路运输动物携带甲型流感病毒进行快速检测的可行性,为制定铁路系统有效的流感监测方案奠定基础。方法采集死禽咽喉部和泄殖腔拭子,活体动物用拭子涂抹泄殖腔外周,粪便和毛用拭子涂抹表面。依据《全国流感监测实施方案》、甲型流感病毒核酸检测试剂盒(PCR-荧光探针法)说明书以及ABI7500F型实时荧光定量PCR的SDS软件操作。结果共采集广州中铁快运货场动物样本250份,检出甲型流感病毒核酸阳性11份,阳性率4.4%,其中以鸽类样本的阳性检出率最高,鹅次之,鸭最低。结论实时荧光PCR检测方法适合铁路运输的实际要求,结合进一步病毒分型和病毒基因测序,对掌握病毒流行趋势,做好预防控制工作具有重要意义。Objective The feasibility of rapid detection of influenza A virus in animals transported by railway using real-time fluorescent PCR is discussed,and to lay the foundation for working out an effective influenza monitoring plan in railway system.Methods Throat and cloacal swab were collected from dead birds.For living animals,peripheral parts of cloaca smears and coat and manure swabs were collected.Influenza A virus was detected by real-time fluorescence quantitative PCR using ABI 7500 F-type real-time fluorescence quantitative PCR and the result was analyzed by the SDS software and followed the ＂national influenza monitoring scheme＂.Results 250 animal samples were collected in the freight yard of Guangzhou Railway Express,and detected for the nucleic acid of influenza A virus.11 were positive,with a positive rate of 4.4%.Conclusion Real-time fluorescent PCR detection method meets the practical need of rail transport.It is important to grasp the trend of virus prevalent for prevention and control,combining with virus typing and viral gene sequencing.

关 键 词：实时荧光PCR 铁路运输 流感病毒 

分 类 号：R373.13[医药卫生—病原生物学]