中国3种旧大陆猴TRIMCyp嵌合基因的存在状况及基因特性研究  

Investigation and characterization of three kinds of Chinese old world monkey TRIMCyp genotype

作　　者：那雷[1] 于长清[2] 季芳[3] 汤艳东[2] 李亮[1] 林跃智[2] 吕晓玲[2] 刘晓明[3] 郑永辉 周建华[2] 曲娟娟[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室,黑龙江哈尔滨150001 [3]广东省昆虫研究所华南灵长类研发中心,广东广州510030 [4]Microbiology and Molecular Genetics,Michigan State University

出　　处：《中国预防兽医学报》2011年第8期615-619,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：十二五"重大新药创制"科技重大专项(2011ZX09307-303-03);广东省科技计划项目(2010B060500023);国家自然科学面上项目(31070809);兽医生物技术国家重点实验室开放基金(SKLVBF200904)

摘　　要：本研究应用PCR方法检测了中国3种旧大陆猴(恒河猴、食蟹猴及藏酋猴)的TRIMCyp嵌合基因型个体的存在状况,并首次发现携带TRIMCyp嵌合基因中国品系恒河猴。同时本实验应用建立RT-PCR方法在mRNA水平上进一步检测了恒河猴和食蟹猴的TRIMCyp嵌合基因序列和特性。其编码的融合蛋白TRIMCyp丧失了对HIV-1复制的限制功能,推测具有该基因型旧大陆猴对HIV-1更易感,可用于建立新型的艾滋病非人灵长类动物模型。本研究结果将促进逆转录病毒跨种间传播的研究和建立HIV-1易感非人灵长类动物模型。Based on recent publications, TRIMCyp, the fusion protein of TRIM5 a and CypA, lost its ability to restrict the replication of some retroviruses in the old world monkeys, such as human immunodeficiency virus （HIV-1）. However, there are no reports on the distribution of TRIMCyp chimera gene in major old world monkeys, except Pig-tailed Macaque in China. In addition, several published investigations failed to find TRIMCyp gene in Chinese Rhesus Macaque. In this study, the existence of TR/MCyp, the TRIM5 gene with a retrotransposon CypA cDNA, in 3 major Chinese old world monkeys （Rhesus Macaque, Crab-eating Macaque and Thibetana Macaque） was investigated by PCR. For the first time, the TRIMCyp chimera gene was found existing in a small population of Chinese Rhesus Macaque. Further more, a RT-PCR protocol was developed to detect the mRNA of TRIMCyp in Rhesus Macaque and Crab-eating Macaque. Macaque with this chimera gene are considered susceptible to HIV-1since the TRIMCyp fusion protein does not effectively inhibit the replication of HIV-1. The TRIMCyp/TRIMCyp genotype Macaques have the potential to be developed as novel non-human primate （NHP） animal models for HIV researches. Our results will benefit the studies on cross-species transmission of retrovimses and the development of novel H1V-l-susceptible NHP models.

关 键 词：RT-PCR 中国旧世纪猴 TRIMCyp HIV-1 

分 类 号：S852.65[农业科学—基础兽医学]