Association between SNP rs1800875,serum chymase and immunoglobulin E levels in patients with coronary heart disease  

作　　者：Chun-na JIN Hong MA Yan LIN Jian-an WANG Mei-xiang XIAN 

机构地区：[1]Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China [2]Cardiovascular Key Lab of Zhejiang Province, the Second Affiliated Hospital School of Medicine, Zhejiang University, Hangzhou 310009, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2011年第8期660-667,共8页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China (No. 30670867) to Mei-xiang XIANG;the Major Program of Science and Technology Department of Zhejiang Province,China(No. 2007C13058) to Mei-xiang XIANG

摘　　要：Objective:The gene for mast cell chymase (CMA1) is an ideal candidate for investigating the genetic predisposition to coronary heart disease (CHD),as activated mast cells have been found to be present in a greater proportion in the shoulder region of atheroma than in normal coronary intimae.Previous studies have indicated that CMA1 promoter polymorphism rs1800875 may be involved in regulating immunoglobulin E (IgE) levels in patients with eczema,and it is associated with the progression of immunoglobulin A nephropathy.Methods:The association between single nucleotide polymorphism (SNP) rs1800875,serum chymase,and serum IgE levels was examined in 175 CHD subjects and 95 non-CHD subjects.Results:Statistical analysis indicated no significant difference in allele frequency between CHD and non-CHD.However,a significant association was found between CMA1 genotypes and total IgE levels in CHD subjects.Meanwhile,crossover analysis revealed that,in GG homozygotes,CHD risk was nearly six times higher in those with IgE (U/ml) level <2.58 (natural logarithm conversion),while no association was found with chymase level.Conclusions:Polymorphism rs1800875 of CMA1 may be associated with serum IgE level in CHD subjects,but not with chymase level in both groups.In GG homozygotes,high IgE level is a protective factor against coronary disease.Objective： The gene for mast cell chymase （CMA1） is an ideal candidate for investigating the genetic predisposition to coronary heart disease （CHD）, as activated mast cells have been found to be present in a greater proportion in the shoulder region of atheroma than in normal coronary intimae. Previous studies have indicated that CMA 1 promoter polymorphism rs1800875 may be involved in regulating immunoglobulin E （IgE） levels in patients with eczema, and it is associated with the progression of immunoglobulin A nephropathy. Methods： The association between single nucleotide polymorphism （SNP） rs1800875, serum chymase, and serum IgE levels was examined in 175 CHD subjects and 95 non-CHD subjects. Results： Statistical analysis indicated no significant difference in allele fre- quency between CHD and non-CHD. However, a significant association was found between CMA1 genotypes and total IgE levels in CHD subjects. Meanwhile, crossover analysis revealed that, in GG homozygotes, CHD risk was nearly six times higher in those with IgE （U/ml） level 〈2.58 （natural logarithm conversion）, while no association was found with chymase level. Conclusions： Polymorphism rs1800875 of CMA1 may be associated with serum IgE level in CHD subjects, but not with chymase level in both groups. In GG homozygotes, high IgE level is a protective factor against coronary disease.

关 键 词：Coronary heart disease CMA 1 Serum immunoglobulin E （IgE） Serum chymase 

分 类 号：R543.3[医药卫生—心血管疾病]