黄芩苷对自身免疫性脑脊髓炎大鼠细胞凋亡的影响  

Effects of baicalin on apoptosis in rats with autoimmune encephalomyelitis

作　　者：徐俊[1] 黄榕[1] 杨于嘉[1] 金世杰[2] 张金凤 

机构地区：[1]中南大学湘雅医院儿科,湖南长沙410008 [2]湖南省儿童医院,湖南长沙410008 [3]广东省佛山市顺德区妇幼保健院,广东佛山528000

出　　处：《中国当代儿科杂志》2011年第8期665-668,共4页Chinese Journal of Contemporary Pediatrics

摘　　要：目的观察黄芩苷(BAC)对自身免疫性脑脊髓炎(EAE)大鼠的疗效及其对脊髓炎症细胞凋亡的影响。方法 44只Wistar大鼠随机分为正常对照组(NC,n=10)、EAE组(n=12)、地塞米松治疗组(DXM,n=12)、BAC治疗组(n=10)。DXM组和BAC组在抗原免疫后1周分别予以DXM(1 mg/kg)和BAC(200 mg/kg)治疗7 d;观察各组动物脊髓病理变化及髓鞘碱性蛋白(MBP)表达的情况和脊髓炎症细胞凋亡情况。结果 EAE组、DXM组和BAC组较NC组体重均下降,BAC组体重较EAE组及DXM组明显上升(P<0.05)。DXM组和BAC组大鼠神经功能评分明显优于EAE组(P<0.05);DXM组和BAC组脊髓MBP阳性数均较EAE组显著增多(P<0.05)。DXM组和BAC组脊髓炎症细胞凋亡数均较EAE组显著增多(P<0.05)。结论 BAC对EAE大鼠有治疗作用,并且能促进大鼠脊髓炎症细胞凋亡。Objective To study the therapeutic efficacy of baicalin and its effect on apoptosis of inflammatory cells in spinal cords in Wistar rats with autoimmune encephalomyelitis（EAE）. Methods Forty-four rats were randomly divided into four groups： normal control group（control,n=10）,EAE group（n=12）,and two intervention groups with dexamethasone（DXM） or baicalin.Seven days after immunization,the two intervention groups were injected intraperitoneally with DXM（1 mg/kg） and baicalin（200 mg/kg） for 1 week,respectively.The spinal cords were removed 14 days after immunization,and stained with hematoxylin and eosin.MBP expression in spinal cords was detected by immunohistochemistry.The apoptosis of inflammatory cells in spinal cords was detected by TUNEL. Results The weight gain rate in the untreated EAE and the DXM or baicalin intervention groups were significantly lower than that in the control group（P0.05）.The weight gain rate in the baicalin intervention group was significantly higher than that in the untreated EAE and the DXM intervention groups（P0.05）.The scores of neurological function in the two intervention groups were significantly higher than that in the untreated EAE group（P0.05）.DXM or baicalin treatment significantly increased the MBP expression compared with the untreated EAE group（P0.05）.The apoptosis of inflammatory cells increased more in the DXM and the baicalin intervention groups compared with the untreated EAE groups（P0.05）. Conclusions Baicalin has protective effects against EAE in rats.It can promote the apoptosis of inflammatory cells in spinal cords.

关 键 词：实验性自身免疫性脑脊髓炎 细胞凋亡 地塞米松 黄芩苷 大鼠 

分 类 号：R285.5[医药卫生—中药学]