聚乙烯醇／壳聚糖复合纳米纤维支架构建及降解行为研究  

Preparation of polyvinyl alcohoi/chitosan hybrid nanofibrous scaffolds and study on its biodegrada- tion behaviors in vivo and in vitro

作　　者：牛震海[1] 金正花[1] 吴巍巍[1] 关宏宇[2] 石凯[1] 赵景春[1] 刘继强[1] 于家傲[1] 

机构地区：[1]吉林大学第一医院烧伤外科,长春130031 [2]东北师范大学化学学院

出　　处：《中华烧伤杂志》2011年第4期269-273,共5页Chinese Journal of Burns

基　　金：吉林省科技发展计划（200905132）

摘　　要：目的制备能够模拟皮肤ECM生理结构的聚乙烯醇（PVA）／壳聚糖复合纳米纤维支架，并研究其体内外降解行为。方法（1）利用静电纺丝技术分别构建PVA纳米纤维支架和PVA／壳聚糖复合纳米纤维支架，采用戊二醛蒸气法对2种支架样本分别进行交联，于扫描电镜下观察2种支架的形貌。（2）体外降解实验：分别裁取2种支架样本（2cm×2cm）置于PBS中，37．0℃水浴温育3、7、14d墩出，干燥，真空条件下喷金后于扫描电镜下观察支架形貌变化。（3）体内降解实验：将48只Wistar大鼠按随机数字表法分为2组，其一为PVA组，大鼠脊柱两侧对称区域（4处）皮下埋植PVA纳米纤维支架；另一为PVA／壳聚糖组，大鼠脊柱两侧对称区域（4处）皮下埋植PVA／壳聚糖复合纳米纤维支架。每组24只大鼠。术后3、7、14、28d，分别取各组6只大鼠埋植样本，HE染色行组织形态学观察。结果（1）交联后PVA纳米纤维支架和PVA／壳聚糖复合纳米纤维支架的纤维表面光滑，纤维直径分布均匀（200～300nm），具有较高的孔隙率。（2）体外降解实验结果显示：温育3、7、14d，PVA纳米纤维支架的纤维分别呈现溶胀、溶解、融合的形貌变化；而PVA／壳聚糖复合纳米纤维支架的纤维则分别呈现溶胀、溶解断裂、消失的形貌变化。（3）体内降解实验结果显示：术后3、7、14、28d，2组纤维支架结构均分别呈现松散、边界不清、大部分被降解及最终消失的形貌学变化。结论采用静电纺丝技术可成功制备PVA／壳聚糖复合纳米纤维支架，交联后的该支架具有与组织雨构相适宦的降解行为。Objective To fabricate polyvinyl alcohol （PVA）/chitosan hybrid nanofibrous seaffohts owning the similar physiological structure of ECM, and to observe its biodegradation behavior in vivo and in vitro. Methods （1） The PVA nanofibrous scaflold and PVA/chitosan hybrid nanofibrous scaffold were fabricated by electrospinning technique, and then they were crosslinked by glutaraldehyde vapor method. The morphology of both scaffolds was observed by scanning electron microscope （ SEM ）. （2） Biodegradation experiment in vitro： the samples of two scaffolds with size of 2 cm×2 cm were placed into phosphate-buffer saline （PBS） fluid under 37.0 ℃ water for incubation, and then they were dried to observe morphologic changes underSEM on post incubation day （PID） 3, 7, and 14. （3） Biodegradation experiment in vivo： 48 ／Vistar rats were divided into PVA group and PVA/chitosan group according to the random number table, with 24 rats in each group. PVA or PVA/chitosan nanofibrous seaftbld was implanted into subcutaneous tissue on bnth sides of back in rats of both groups, with 4 scafh）lds in each rat. The scaffold samples were harvested to observe morphologie changes with HE staining on post operation day （POD） 3, 7, 14, and 28. Results （ 1 ） After crosslinking, the surface of fibers in PVA and PVA/chitosan hybrid nanofibrous scaffolds were smooth, and the diameters of fibers were similar, ranging from 200 to 300 nm, with high porosity. （2） Biodegradation experiment in vitro showed that the morphologic changes in fiber was respectively swelling, dissolution, fusion in PVA nanofibrous scaffold on PID 3, 7, 14, and that in PVA/chitosan hybrid nanofibrous scaffold was respectively swelling, dissolution and fragmentation, and disappearance. （3） Bio- degradation experiment in vivo showed that the morphulogie changes in scaffold structure was respectively loosening, fuzziness of edges, degradation, and disappearance in PVA group and PVA/chitosan group on POD 3, 7, 14, 28. Conclusions

关 键 词：组织工程 壳聚糖 纳米结构 聚乙烯醇 材料检测 降解 

分 类 号：R318.08[医药卫生—生物医学工程]