静磁场作用下金属离子对成骨细胞的生物学影响  

The Bio-Effect of Metal Ions on Osteoblasts under the Static Magnetic Field

作　　者：戴闽[1] 周通华[1] 程细高[1] 熊皞[1] 邹文楠[2] 黄小琴 

机构地区：[1]南昌大学第一附属医院骨二科,330006 [2]南昌大学建筑工程学院工程力学实验中心 [3]东乡县中医院

出　　处：《天津医药》2011年第8期727-729,777,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30760255);南昌大学学科交叉基金(项目编号:Z03722)

摘　　要：目的:探讨静磁场(SMF)作用下金属离子对成骨细胞的毒性影响,寻找防治假体周围骨溶解的理论依据。方法:将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液,将Co2+、Cr3+分别与小鼠颅骨成骨细胞(MC3T3-E1)在不同的磁场强度下共培养。将细胞随机分为对照组及3个实验组,各实验组分别放入1、10和100mT的磁场中,培养12、24和48h。倒置显微镜下观察细胞生长情况及形态特征。扫描电镜检测成骨细胞表面超微结构。流式细胞术检测细胞凋亡情况。酶联免疫吸附测定(ELISA)法对培养上清液碱性磷酸酶(ALP)含量进行检测。结果:静磁场作用24h后的成骨细胞呈现更加成熟的形态学特征,不同强度静磁场加载组的成骨细胞细凋亡率除1mT组在12和24h与对照组比较差异无统计学意义外,其他各组不同时段凋亡率均小于对照组。100mT组ALP活性明显高于对照组。结论:静磁场作用下Co2+、Cr3+金属离子对成骨细胞的毒性作用会明显减低,为磁场疗法防治假体周围骨溶解提供了实验依据。Objective：To investigate the toxic effect of metal ions Co2＋ and Cr3＋ on osteoblast under the static magnetic field,and search the method for the protection and treatment of aseptic loosening.Methods：Coalt chloride powder and CrCl3 powder were dissolved in the asepsis injecting water.The mouse calvarial osteoblasts（MC3T3-E1） were cultured with Co2＋,Cr3＋ ions in different magnetic fields of 1,10 and 100 mT for 12,24 and 48 h.The morphological features of cell growth were observed under inverted microscope.The ultrastructure of osteoblasts was detected by scanning electron microscopy.The cell apoptosis rate was assured by flow cytometry.ELISA method was applied to detect alkaline phosphatase（ALP）content in serum supernatant.Results：Under the static magnetic field,osteoblasts showed more mature morphological features.There were no significant differences in the apoptosis rate of osteoblasts under different static magnetic fields except for the group of 1 mT at 12 and 24 h compared with those of control.The apoptosis rate was lower in group 1,10 and 100 mT compared with that of experiment group.The value of ALP was significantly higher in 100 mT group than that of control.Conclusion：The static magnetic field could reduced the toxic effect of metal ions Co2＋ and Cr3＋ on osteoblasts,the offered the experimental evidence for the protection and treatment of aseptic loosening with magnetic field therapy.

关 键 词：电磁场 成骨细胞 钴铬 假体失效 细胞 培养的 

分 类 号：R683.5[医药卫生—骨科学]