周围神经慢性卡压损伤过程中转化生长因子β1和Ⅰ、Ⅲ型胶原的含量及相关性分析  

Effects of transforming growth factor [~ and collagen type Ⅰ/Ⅲ on chronic peripheral nerve compression injury

作　　者：胡锐[1] 劳杰[2] 贾中尉[1] 孟繁斌[1] 陈振兵[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院手外科,武汉430022 [2]复旦大学附属华山医院手外科,上海

出　　处：《中华手外科杂志》2011年第4期223-226,共4页Chinese Journal of Hand Surgery

基　　金：基金项目：国家自然科学基金资助项目（30872627）;上海市周围神经显微外科重点实验课题（08Dz2270600）

摘　　要：目的探讨转化生长因子岛（TGF-β1）和Ⅰ、Ⅲ型胶原蛋白（COL-Ⅰ、COL-Ⅲ）在周围神经慢性卡压中的作用及二者的相关性。方法将50只成年雄性SD大鼠随机分成2组：对照组，仅分离暴露坐骨神经；卡压组，对大鼠右侧后腿坐骨神经行硅胶管卡压术。于卡压术后2．4、6、8、10周随机取2组大鼠各5只，切取卡压段神经行组织形态学、免疫组织化学观察及RT-PCR、WesternBlot定量测定TGF-β1及COL-Ⅰ、COL-Ⅲ含量。结果坐骨神经慢性卡压损伤后，神经内胶原纤维增生；卡压组大鼠坐骨神经TGF-β1及COL-I、COL-Ⅲ含量明显高于对照组，两者差异有统计学意义（P〈0．05）；TGF-β1与COL-Ⅰ、COL-Ⅲ含量在一定时期内呈正相关。结论慢性卡压损伤可致周围神经发生纤维化病变，COL-Ⅰ、COL-Ⅲ含量显著增加，TGF-β1参与了这一病理生理改变过程，提示TGF-β1在周围神经纤维化过程中具有一定的作用。Objective To investigate the effects of transforming growth factor β1（TGF-β1） and collagen type Ⅰ/Ⅲ （COL- Ⅰ/Ⅲ ） on chronic peripheral nerve compression injury and explore the relationship between TGF-β1 and COL- Ⅰ/Ⅲ. Methods Fifty adult male SD rats were randomly divided into group A and B. In group A （sham-operated group） the sciatic nerve was exposed only. In group B （compression group） sciatic nerve entrapment was created on the right hind leg according to Mackinnon＇ s procedure of chronic sciatic nerve compression. Electron microscopy, inrmunohistechemistry, RT-PCR and Western-Blot were performed to observe the morphological changes of the compressed nerve tissue and to determine the level of TGF-~, collagen- Ⅰ and collagen-Ⅲ 2, 4, 6, 8 and 10 weeks after the surgery, respectively. Results After sciatic nerve compression, collagen content in the nerve increased. The expression of TGF-β1 and COL- Ⅰ/Ⅲ in the sciatic nerve of the compressed group increased, which was significantly higher than that of the sham-operation group （ P 〈 0.05）. In the meanwhile, the contents of TGF-β1 and COL- Ⅰ/Ⅲ were positively correlated in a certain period. Conclusion Peripheral nerve fibrosis can he caused by chronic nerve compression which induces increased expression of COL-Ⅰ/Ⅲ in the nerve. TGF-I~ is involved in the pathophysiolagical process, which suggests that TGF-β1 plays an important role in the process of neural injury and fibrosis.

关 键 词：周围神经 神经卡压综合征 转化生长因子-Β Ⅰ/Ⅲ型胶原蛋白 

分 类 号：R651.3[医药卫生—外科学]