钛颗粒负荷对成骨细胞释放细胞因子的影响  

Influence of Titanium Particles Loading on Releases of Cytokines of Osteoblasts

作　　者：吴江[1] 王艳霞[2] 任弘毅[1] 孙亨[1] 范恒[1] 王海舟[1] 陈槐卿[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物医学工程研究室,成都610041 [2]四川大学图书馆,成都610041

出　　处：《生物医学工程学杂志》2011年第4期748-752,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10802054)

摘　　要：假体周围的溶骨性因子是造成无菌性松动的主要原因之一。前期的研究表明,磨损颗粒抑制成骨细胞分化和矿化能力,为探索不同直径的磨损颗粒对成骨细胞释放细胞因子的影响,我们采用双夹心抗体ELISA、RT-PCR法分析3种直径钛颗粒对兔成骨细胞表达IL-6I、L-10和破骨细胞分化因子(ODF)的影响。结果显示:0.9μm钛颗粒刺激成骨细胞产生大量促骨吸收因子(IL-6、ODF)的同时快速而短暂地释放抑骨吸收因子(IL-10);2.7μm和6.9μm钛颗粒,尤其是后者主要是刺激成骨细胞缓慢而强烈地产生促骨吸收因子。提示:磨屑颗粒作用成骨细胞的生物学反应是双相的,且反应程度因颗粒大小和作用时间的不同而又有所不同。可见如何抑制假体周围溶骨性因子的产生以及人工材料的优化选择是提高人工假体寿命的关键。Aseptic loosening is mainly mediated by bone resorption cytokines in the surrounding area of orthopaedic implants.Our previous investigation demonstrtated that different-sized titanium particles loading can inbibit the osteoblastic differentiation and mineraliztion.In order to investigate the hypothesis that particulate wear debris derived from prosthetic biomaterials affects the release of bone resorption related cytokines,we studied the influence of differentsized titanium particles loading on the osteoblastic cytokines by assaying the secretion of IL-6、IL-10 with use of ABC-quantitative sandwich enzyme-linked immunosorbent assay,and on the expression of osteoclast differentiation factors（ODF） by RT-PCR.The results showed that the 0.9μm titanium particles promoted osteoblasts producing bone resorption cytokines（IL-6,ODF）,and simultaneously secreted bone absorption restraining factor（IL-10） quickly and transitorily.In comparison,the 2.7μm and 6.9μm titanium particles,especially the latter primarily promoted osteoblasts secreting bone absorption promoting factors powerfully and slowly.The results suggested that there was a biphasic response appearing in titanium particles loaded-osteoblastic cultures,the level of which varied according to the different size and the loading time of titanium particles.This in vitro experimental result showed that attentaion to the inhibition of bone resorption cytokines stimulated by wear debris and to the screen potential favourable biomaterials for implants must be taken.

关 键 词：钛颗粒 成骨细胞 破骨细胞 破骨细胞分化因子 

分 类 号：R318.08[医药卫生—生物医学工程]