多肽自组装纳米纤维凝胶与大鼠嗅鞘细胞相容性的实验研究  

Experimental Research on the Compatibility of Self-assembly Nanofiber Hydrogel from the Amphipathic Peptide Containing IKVAV with Olfactory Ensheathing Cells of Rats

作　　者：朱乐银[1] 崔志明[1] 徐冠华[1] 朱志康[2] 黄镇[2] 保国峰[1] 孙郁雨[1] 王玲玲[1] 崔颖[1] 

机构地区：[1]南通大学第二附属医院脊柱外科,南通226001 [2]南通大学神经生物学研究所,南通226001

出　　处：《生物医学工程学杂志》2011年第4期774-779,共6页Journal of Biomedical Engineering

基　　金：江苏省六大人才高峰基金资助项目(06-B-053);江苏省医学重点人才基金资助项目(R2007027)

摘　　要：为了研究自组装纳米纤维凝胶材料异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸(IKVAV)多肽与大鼠嗅鞘细胞(OECs)的生物相容性。将培养纯化的大鼠OECs种植于自组装制成IKVAV多肽纳米纤维凝胶表面(二维培养)和纤维凝胶内部(三维培养),倒置显微镜和免疫荧光镜下观察OECs的黏附、增殖情况,并采用MTT法和S-100阳性细胞计数、胞体面积、细胞周长的图像处理和统计分析检测支架对细胞的毒性来评价细胞的活力。OECs接种到IKVAV凝胶后,大多数OECs能在凝胶中生长良好,MTT检测及荧光显微镜下观察OECs的数目、周长与面积与多聚赖氨酸(PLL)组无显著性差异。证实自组装IKVAV多肽纳米纤维凝胶与大鼠OECs有良好的生物相容性。The present research was aimed to explore the biocompatibility of IKVAV self-assembling peptide nanofiber scaffold with olfactory ensheathing cells（OECs） of rats.The OECs were seeded onto the surface of coverslips covered with IKVAV self-assembling peptide nanofiber scaffold hydrogel（2D culture system）,and implanted within IKVAV self-assembling peptide nanofiber scaffold hydrogel（3D culture system）,respectively.The adhesion,viability of OECs were observed with inverted microscope.Then the characteristics for survival and adhesion of cells by image processing were observed,and statistical analysis on the number of S-100 positive cel1,the area of the cell bodies and the perimeter of the cell and MTT method were carried out.It was found that the OECs could survive and migrate in IKVAV self-assembling peptide nanofiber scaffold.The result of the cell MTT exam,of the shape and quantity of cells had no significant difference compared to those of the OECs cultured with poly-L-lysine（PLL）.It has been proved that IKVAV self-assembling peptide nanofiber scaffold has good biocompatibility with rat OECs.

关 键 词：嗅鞘细胞 纳米纤维 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]