血红素氧合酶-1/一氧化碳和一氧化氮合酶/一氧化氮系统对球囊损伤后血管重塑的影响及其两系统的相关性研究  

Influences of Heme Oxygenase-1,Carbon Monoxide and Nitricoxide Synthase,Nitrogen Monoxide Systems on Vascular Remodeling of Injured Balloon Carotid Artery in Rabbits and the Intercorrelations among the Two Systems

作　　者：刘大男[1] 何作云[2] 吴立荣[1] 方颖[1] 刘兴德[1] 李屏[1] 

机构地区：[1]贵阳医学院附属医院心内科,贵阳550004 [2]第三军医大学新桥医院心内科,重庆400037

出　　处：《生物医学工程学杂志》2011年第4期790-794,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(39800065);贵州省科学基金资助项目[黔科合字(2006)2074号];贵州省优秀科技教育人才省长资金项目[黔省专合字(2007)72号]

摘　　要：探讨球囊损伤后血管重塑中血红素氧合酶-1(HO-1)/一氧化碳(CO)系统与一氧化氮合酶(NOS)/一氧化氮(NO)系统的作用及相互关系。家兔随机分为对照组、假手术组、胆固醇组、精氨酸组、亚硝基组、血红素组和卟啉锌组。对照组喂普通饲料,其余六组喂含1.5%胆固醇饲料,精氨酸组和亚硝基组饮水同时予L-精氨酸或亚硝基-L-精氨酸甲酯,血红素组和卟啉锌组同时分别予氯化血红素或锌原卟啉-9腹腔内注射。2周后实验组行一侧颈总动脉球囊损伤,术后续原喂养给药8周。结果显示:与胆固醇组比较,血红素组HO活性、CO生成量显著增加,核因子κB活性显著降低,内膜面积显著减小(均P<0.01);卟啉锌组呈现相反的结果;精氨酸组cNOS活性、NO生成量显著增加,核因子κB活性显著降低,内膜面积显著减小(均P<0.01);亚硝基组cNOS活性、NO生成量显著降低,核因子κB活性显著升高(均P<0.01),内膜面积变化无显著性。本研究表明,再狭窄形成中HO-1/CO与NOS/NO两系统显示互补及代偿作用,HO-1/CO系统可能通过对NOS/NO系统的代偿和调节并抑制核因子κB活性,从而抑制血管损伤后内膜增殖及负性重塑。The aim of this study was to investigate the influences of heme oxygenase-1,carbon monoxide and nitricoxide synthase,nitrogen monoxide systems on vascular remodeling of injured balloon carotid artery in rabbits and the intercorrelations among the two systems after balloon angioplasty.Seventy rabbits were randomly divided into seven groups,i.e.,control group,SH group,Chol group,Arg group,L-NAME group,Hem group,and Znpp group.The control group received normal chow,while all the rabbits the rest six groups received 1.5% cholesterol diet.Among the six test groups,to those in Chol group and SH group nothing else was added except the 1.5% cholesterol.L-arginine or L-nitro-arginine methylester was added to those in the Arg group and in the L-NAME group with drinking water.Hemin or zincprotoporphyrin IX was added to those in Hem group and in Znpp group by injecting the medicine into the abdominal cavity.After two weeks,the experimental groups underwent balloon injury at one side common carotid artery.Compared to Chol group,the HO-1 activity and CO production increased significantly.The intima area was reduced distinctly in Hem group,while there were opposite results in Znpp group.Compared with that in Chol group,the NF-кB activity of Arg group and Hem group were lower significantly.That of L-NAME group and Znpp group were higher significantly.Compared with that in the Chol group,the cNOS activity and NO production were eleveated markedly in Arg group while they were decreased markedly in L-NAME group.The intima area was reduced significantly in Arg group,while in L-NAME group they were not different from those in Chol group.These results suggested that the reciprocal relationship between HO-1/CO and NOS/NO system in restenosis may play the inhibitory role against neointimal proliferation and vascular wall remodeling after balloon angioplasty.

关 键 词：动脉 球囊损伤 血管重塑 一氧化碳 一氧化氮 

分 类 号：R543.5[医药卫生—心血管疾病]