磷酸钙骨水泥／聚磷酸钙纤维复合不同配比的颗粒骨修复兔桡骨缺损  

Repair of rabbit bone defect with composite of calcium phosphate bone cement and calcium phosphate cement mixed with different ratio of minimal morselized bone in vivo

作　　者：马佳滨[1] 周磊[1] 闫景龙[1] 昌广平[2] 潘华兴[1] 奚春阳[1] 祖佳宁[1] 姬烨[1] 李燕京[3] 关智慧 

机构地区：[1]哈尔滨医科大学附属第一医院骨科,150001 [2]哈尔滨医科大学附属第一医院ICU,150001 [3]哈尔滨医科大学附属第三医院肿瘤内科 [4]哈尔滨市儿童医院麻醉科

出　　处：《中华创伤杂志》2011年第8期737-741,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（30672126）;黑龙江省卫生厅科研课题资助项目（2009-030）;哈尔滨市科技创新人才研究专项资金资助项目（2009RFOXS222）

摘　　要：目的探讨不同纤维配比的复合人工骨修复兔桡骨缺损的成骨效果。方法选用新西兰大白兔36只，完全随机化设计分为A、B、C、D四组，将其双侧桡骨制备成骨缺损模型，取其自体髂骨制成直径为300～500μm的微小颗粒骨，将磷酸钙骨水泥（calcium phosphate cement，CPC）／颗粒骨与聚磷酸钙纤维（calcium polyphosphate fiber，CPPF）均匀混合成CPC／CPPF复合材料，按照CPPF占CPC／CPPF复合材料重量的0、10％、30％、50％分为四组，将上述四组CPC／CPPF复合材料与微小颗粒骨以6：4固定比例混合均匀，分别植入A、B、C、D四组兔桡骨缺损处，在4，8周分别行大体、X线片和组织学观察，8周时行力学测试。结果当CPPF占CPC／CPPF复合材料的30％时，最大抗压负荷及抗弯负荷均优于其他组（P〈0．05），组织学显示人工复合材料与骨组织界面结合最为紧密，材料降解速率与组织成骨速率最接近，成骨性最佳，比例最优。结论通过向CPC／CPPF中加入一定比例颗粒骨来调节复合材料降解速率，使之与兔桡骨成骨速率相适应，以获得修复兔桡骨缺损的最佳复合人工骨。Objective To explore the effect of the calcium phosphate cement （CPC） /caMum polyphosphate fiber （CPPF） composites mixed with different proportion of minimal morselized bone on repair of bone defect in vivo. Methods A total of 36 New Zealand white rabbits were completely randomly designed into A, B, C, D groups and their bilateral radial bone defect model was prepared. The minimal morselized bone （300-500 μm in diameter） was made from the iliac of those rats. The CPPF and CPC were evenly mixed into CPC/CPPF composites which were divided into four groups in accordance with the CPPF weight 0, 10% , 30% and 50% in CPC/CPPF composite. The CPC/CPPF composites of the four groups was mixed with the minimal morselized bone with ratio of 6：4 and then the mixture was implanted the bone defect of the rabbits in four groups. The gross, X-ray and histological observations were done at four and eight weeks. The biomeehanical test was performed at eight weeks. Results When CPPF occupies 30% of the CPC/CPPF composite, the maxiumm compressive load and bending loads were better than those in the other groups （P 〈 0.05 ） , when the histological observation showed the most tight link between the artificial composite and the bone interface and the closest similarity between material degradation rate and the ossification rate, with the best osteogenesis and the optimal ratio. Conclusion The repair of bone defect can attain the optimal outcome through adding a certain ratio of minimal morselized bone into the CPC/CPPF to adjust the degradation rate of composites.

关 键 词：磷酸钙类 骨移植 骨替用物 磷酸钙骨水泥 

分 类 号：R318.08[医药卫生—生物医学工程]