应用水稻基因芯片分析小麦光温敏核雄性不育系的基因差异表达  

Gene Differential Expression of Photo-thermo Sensitive Genic Male Sterile Line by using Rice Microarray

作　　者：张立平[1,2] 许晨光[1,2] 赵昌平[1,2] 张风廷[1] 单福华[1] 苑少华[1] 

机构地区：[1]北京市农林科学院北京杂交小麦工程技术研究中心,北京100097 [2]首都师范大学生命科学学院,北京100048

出　　处：《中国生物化学与分子生物学报》2011年第8期761-767,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：北京市自然科学基金(No.5091001);国家高技术研究与发展计划(863)(No.2011AA10A106);农业部948项目(No.2009-Z4);院创新能力建设专项(No.KJCX201101007);北京农业育种基础研究创新平台项目(No.D08070500690801)~~

摘　　要：小麦光温敏雄性不育系是二系法杂交小麦应用技术体系的核心与基础,其育性严格受光周期和温度控制.了解光温敏雄性不育机理将促进二系法杂交小麦育种技术的应用和发展,而筛选控制不育的关键基因是揭示光温敏雄性不育分子机理的前提.由于小麦基因组信息有限,根据小麦与水稻有较高同源性,尝试利用水稻基因组芯片筛选小麦光温敏雄性不育系BS366冷胁迫响应基因.得到9个差异表达基因,这些基因参与基因表达调控、胁迫应答、信号转导、代谢等重要生命过程,为解析小麦光温敏雄性不育机理提供了有益信息.利用雄蕊cDNA半定量PCR法验证表明,NADH(nicotinamide adenine dinucleotide hydrate)脱氢酶亚基4L、锌指富含DHHC(deaf/hard ofhearing connection)结构、线粒体物质运输蛋白、外被体蛋白COPⅠδ(coat proteinⅠδ)亚基和ABC(ATP-binding cassette)转运蛋白5个基因,在低温和对照温度下表达存在明显差异,可作为育性相关候选基因开展下一步研究.Photo-thermo-sensitive genic male sterile（PTGMS） line is an outstanding alternative for the two-line hybrid system in crop breeding,in which fertility is strictly controlled by photoperiod and temperature.To investigate the molecular mechanism of PTGMS that facilitates its application and the hybrid system development,it is essential to find out the key genes to control the fertility alteration.We used a rice gene microarray to screen for the genes of male fertility alteration in the wheat PTGMS line BS366 based on the homology between wheat and rice.We found 9 candidate genes involved the biological process of gene expression regulation,stress responses,signal transduction and metabolism.The reliability of the genechip result was verified by semi-quantitative PCR.Five of the candidate genes,NADH dehydrogenase subunit 4L,zinc finger DHHC（deaf/hard of hearing connection）domain-containing protein,mitochondrial substrate carrier family protein,COPⅠδ（coat proteinⅠδ） subunit and ABC（ATP-binding cassette）transporter were to be cloned for the study of their function on fertility alternation in PTGMS wheat.This study may provide new insights for the understanding of fertility alternations.

关 键 词：小麦 光温敏核雄性不育 冷处理 基因芯片 

分 类 号：Q3[生物学—遗传学]