营养性肥胖大鼠下丘脑增食欲素A基因的表达及其意义  

Expression of orexin A and leptin in alimentary obesity rat and its clinical significance

作　　者：赵玉岩[1] 王露[1] 马东杰[1] 田蕾[1] 郑德禄[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,沈阳110001

出　　处：《中华老年医学杂志》2011年第8期684-686,共3页Chinese Journal of Geriatrics

基　　金：基金项目：国家自然科学基金资助项目（30872724）;辽宁省教育厅资助科研项目（L20107125、L2010711）;辽宁省科技攻关层次计划项目（2008225009,2009225010-3）

摘　　要：目的探讨营养性肥胖大鼠下丘脑组织中增食欲素A（OrexinA）的表达及其与高瘦素、高胰岛素和高脂血症的关系。方法高脂膳食诱导建立营养性肥胖Wistar大鼠模型；采用放射免疫法和生化酶法检测肥胖大鼠血清瘦素、血清胰岛素及c肽、三酰甘油和总胆固醇；应用实时定量PCR分析法检测肥胖大鼠下丘脑组织中增食欲素A的表达。结果喂养8周时，肥胖组大鼠体质量、Lee’s指数、血糖、三酰甘油及总胆固醇水平较对照组均明显增加（P〈0．01）；肥胖组大鼠血清瘦素（1．74±0．36）ug／L、胰岛素（35±5）MIU／L和c肽含量（0．21±0．04）ug／L，均高于对照组（1．27±0．11）ug／L、（23±4）MIU／L和（0．14±0．03）ug／L，差异均有统计学意义（P〈0．01）；肥胖组大鼠下丘脑组织中增食欲素AmRNA水平较对照组降低，分别为6．8±2．3和14．5±3．6（t=-8．06，P〈0．01）；增食欲素AmRNA水平与血清瘦素（r=-0．726）、胰岛素（r=-0．506）及c肽含量（r=-0．664）显著负相关（均P〈0．01）。结论高脂饮食可诱导Wistar大鼠体内瘦素抵抗和营养性肥胖的发生，营养性肥胖大鼠下丘脑神经元增食欲素A基因的异常表达与瘦素水平及胰岛素抵抗的发生密切相关。Objective To evaluate the relationships of the expression of orexin A in hypothalamus with hyperleptinemia, hyperinsulinism, and hyperlipidemia in alimentary obesity rats. Methods The alimentary obesity rat model was induced by high-fat diet in Wistar rats. The levels of leptin, insulin, C-peptide, total cholesterol （TC） and triglyeride （TG） were detected by luminescent immunoassay and biochemistry enzymic method. The gene expression of orexin A in rat hypothalamus was detected by real-time PCR. The correlation between orexin A and leptin was analyzed. Results After 8 weeks of high-fat diet feeding, the body weight, Lee＇s index, blood glucose, TG and TC significantly increased in obesity rat group （P〈0.01）. The levels of serum leptin, insulin and C-peptide were significantly higher in obesity rats than in controls [（ 1.74 ±0.36） ug/Lvs. （1.27±0.11） ug/L, （35±5） MIU/L vs. （23±4） MIU/L, （0.21±0.04） ug/L vs. （0.14±0.03） ug/L respectively, P〈0.01]. However, the level of orexin A mRNA was lower in obesity rats than in controls （6.8 ± 2.3 vs. 14.5 ± 3.6, t = -- 8.06, P 〈 0.01）. The correlation coefficient （r） of orexin A mRNA with serum leptin, insulin, and C-peptide in alimentary obesity rat were -0. 726 （P〈0.01）, -0. 506 （P〈0.01） and -0. 664 （P〈0.01）, respectively. Conclusions High-fat diet can induce leptin-resistance and alimentary obesity in Wistar rat. The down-regulation of orexin A in rat hypothalamus would be correlated closely to the leptin and insulin resistance in alimentary obesity.

关 键 词：肥胖症 基因表达 

分 类 号：R285.5[医药卫生—中药学]