HOPS复合体蛋白在细胞自噬过程中的功能研究  

Function of HOPS Complex Proteins in Autophagy Process

作　　者：胡晚秋[1] 林志祥[2] 陈丽莲[3] 晏向华[4] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]清华大学生命科学学院,北京100064 [3]中国农业大学生物学院,北京100083 [4]华中农业大学动物科学与技术学院,武汉430070

出　　处：《中国细胞生物学学报》2011年第8期855-860,共6页Chinese Journal of Cell Biology

基　　金：国家十一五科技支撑项目(No.2006BAD07A04)资助项目~~

摘　　要：自噬(autophagy)是一种溶酶体依赖性的细胞内降解途径,其主要功能是将生物大分子(蛋白质、多糖等)或细胞器(线粒体等)回收至溶酶体中并将其降解为单糖、氨基酸等小分子以重复利用。发现HOPS复合体中的两个基因vps39和vps41的缺失会导致酵母内GFP-ATG8大量积累。进一步研究表明,积累的原因是GFP-ATG8与液泡不能发生融合。而在HOPS复合体中的另外两个基因vps16和vps18缺失的情况下,自噬融合没有受到影响;在vps16和vps18双敲除的菌株中,自噬融合同样没有受到影响。该实验结果为理解HOPS复合体的功能和自噬体与液泡融合的过程提供了新的线索。Autophagy is a lysosome dependent intracellular degradation process, which engulfs molecules or organelles to lysosome and then degraded to monosaccharide or amino acid for reuse. We investigated that in HOPS complex genes vps39 and vps41 loss yeasts, there were extensive accumulation of GFP-ATG8, and the fusion processes of autophagy were blocked. But in other two HOPS complex genes vps16 and vps18 loss yeasts, the fusion processes had little change. Moreover, in vpsl6 and vpsl8 double loss cells, the fusion processes were not disturbed. Therefore, our work provided a new insight to understand the function of HOPS complex and the fusion process between autophagosome and lysosome.

关 键 词：自噬 膜泡融合 HOPS复合体 

分 类 号：Q26[生物学—细胞生物学]