Mn-SOD基因导入黄瓜的遗传转化体系  

Establishment of Genetic Transformation System for Cucumber with Mn-SOD Gene

作　　者：范爱丽[1,2] 孙艳[1] 管清美[1] 梁东[1] 

机构地区：[1]西北农林科技大学园艺学院,杨凌712100 [2]广西农业科学院蔬菜研究所,南宁530007

出　　处：《中国细胞生物学学报》2011年第8期897-904,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.40971179)资助项目~~

摘　　要：黄瓜是我国栽培面积最大的喜温蔬菜之一。在生产上，高温干旱等环境胁迫因子严重危害黄瓜的产量和品质，甚至导致绝收。任安芝等综述了SOD与水分、盐分、低温和大气污染等逆境胁迫之间的关系，认为：各种逆境胁迫引起的活性氧代谢平衡失调、生物膜结构破坏是导致植物遭受逆境伤害的机理之一。In this study, several factors affecting transformation rate were studied by Agrobacteriummediated in Nong cheng No.3 cucumber. These factors included sensitivity of cotyledonary nodes to antibiotics （Kan and Cef）, days of pre-culture, concentrations of Agrobacterium tumefaciens, days of co-culture and time of being infected on the optimum culture medium and so on. The high frequency transgenic regeneration system of cucumber was established as follows： cotyledons nodes ofNong cheng No.3 cucumber were pre-cultured for 3 days, inoculated with EHA-105 （D6000.3, AS 10 mg/100 mL） for 5 minutes, then co-cultured for 2 days, transformed to screening-culture medium （50 mg/L Kan, 500 mg/L Cef）, resistant buds grew well in light. 9 resistant plants were obtained and the same positive bands of 658 bp were detected by examining GUS report gene and PCR amplification of Mn-SOD gene. So the integration of the gene into cucumber genome DNA was confirmed in some degree.

关 键 词：黄瓜 Mn—SOD基因 遗传转化 

分 类 号：S642.2[农业科学—蔬菜学] Q943[农业科学—园艺学]