抗氧化剂Isoverbascoside对MGC80-3细胞抗氧化酶活性和癌基因表达的影响  

Effect of Antioxidant Isoverbascoside on Antioxidant Enzyme Activity and Oncogenes Expression of MGC80-3 Cell

作　　者：陈瑞川[1] 李立[2] 苏金华[1] 李忌[1] 王天叫[1] 徐洵[3] 

机构地区：[1]厦门大学抗癌研究中心,厦门361005 [2]厦门大学生物学系,厦门361005 [3]国家海洋局第三研究所

出　　处：《厦门大学学报（自然科学版）》1999年第6期913-917,共5页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金!(C97108)

摘　　要：以苔酚蓝拒染法、核黄素-NBT还原法、DTNB还原法及RNA斑点杂交分析法分别对经不同浓度Isoverbascoside(Isov)处理的MGC80-3 细胞的生长速率、II期抗氧化酶(超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶)活性和N-ras、C-m yc及p53 基因表达作了检测.结果显示:经Isov处理后,细胞生长明显受抑;II期抗氧化酶活性较处理前明显上升;癌基因N-ras、C-m yc的表达较处理前下降,而抑癌基因p53 表达上升. 表明IsovThe grow th ofMGC80-3 cellw as inhibited after treatm entw ith different concentration ofIsoverbascoside from one to six days, respectively. The activities of IIphase antioxidantenzym es(SOD, GSH-Px and CAT) wereup induced w hile MGC80-3 cellw astreat- ed with 10 μm ol/Land 20 μm ol/LIsoverbascoside from tw o to six days. Also, the N-ras and C-m yc oncogenes w ere regulated dow n and the p53 gene was regulated up, which w asdetected by in situ Northern hybridization, aftertreating MGC80-3 cellw ith sam eprocedure. Thesere- sults suggestthatthe inhibition effecton Isoverbascosideon cancercellsm ay beassociated w ith the induce effects on IIphase antioxidantenzym es and its effects on the oncogenes'transcrip- tion regulation.

关 键 词：MGC80-3 抗氧化酶 抗氧化剂 癌基因 Isov 抗癌性 

分 类 号：R286.91[医药卫生—中药学]