23种野生百合遗传关系的SRAP分析  

Phylogenetic Relationship Analysis of 23 Wild Species of Lilium by SRAP Markers

作　　者：智丽[1] 滕中华[2] 李先源[1] 眭顺照[1] 李名扬[1] 

机构地区：[1]西南大学园艺园林学院,重庆市花卉工程技术研究中心,南方山地园艺学教育部重点实验室,重庆400715 [2]西南大学农学与生物技术学院,重庆400715

出　　处：《农业生物技术学报》2011年第4期677-684,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30872063);中央高校基本科研业务费专项资金(No.XDJK2010C077)共同资助

摘　　要：为探讨百合属属下类群间亲缘关系,本研究选用19对SRAP(sequence-related amplified polymorphism)标记引物对百合属(Lilium)23个野生种基因组DNA进行遗传多样性及遗传关系分析;NTSYS-pc2.1和POPGEN1.32数据处理软件分析数据。共得到清晰条带236条,多态性条带233条,多态性条带比率为98.72%,平均每对引物产生12.3条多态性条带。相似系数变化范围为0.3750～0.7679,遗传距离变化范围为0.2642～0.9808,UPGMA聚类结果将23个供试材料分成4类。岷江百合与宜昌百合亲缘关系最近,野百合与朝鲜百合亲缘关系最远,来源相同、形态相似的材料大部分聚在一起,钟花组与卷瓣组存在基因交流。聚类分析结果与形态分类结果大致吻合。SRAP标记适合于百合属植物遗传多样性检验与遗传关系分析。To probe the infrageneric phylogenetic relationship of Lilium,19 sequence-related amplified polymorphism（SRAP） primer combinations were chosen to analyze genetic diversity of 23 wild Lilium species,and NTSYS-pc2.1 and POPGENE1.32 were used for data analysis.Result showed that a total of 236 clear DNA bands were amplified,233 of which were polymorphic,the proportion was 98.72% with average 12.3 polymorphic loci per SRAP primer combination.The genetic similarities ranged from 0.3750 to 0.7679,and the genetic distance ranged from 0.2642 to 0.9808.Twenty three species were classified into four clustering groups by UPGMA.L.regale and L.leucanthum were the mostly closely related systems,whereas L.brownii and L.amabile were the mostly distantly related systems.Materials with similar morphology and from the same source mostly clustered together,and gene exchanges existed between Sect.Lophophorum and Sect.Sinomartagon.The clustering results were basically consistent with morphology classification.The results indicated that SRAP markers are suited to study genetic diversity and phylogenetic relationship of Lilium.

关 键 词：百合属 野生种 SRAP 遗传关系 

分 类 号：S513[农业科学—作物学]