中草药添加剂的抗小鼠腹泻效果及其对小鼠肠道微生物菌群的影响  

Effects of Chinese Medicine Herbal Additives on Anti Bacterial Diarrhea and Intestinal Bacteria of Rats

作　　者：朱雯[1] 张莉[1] 吴跃明[1] 刘建新[1] 

机构地区：[1]浙江大学动物科学学院,农业部动物营养与饲料重点实验室,国家工程实验室,杭州310029

出　　处：《农业生物技术学报》2011年第4期698-704,共7页Journal of Agricultural Biotechnology

基　　金：杭州市科技创新基金(No.20051322B34)资助

摘　　要：为研究中草药提取液的抗小鼠腹泻效果及其对小鼠肠道菌群的影响,本实验选用50只体重为18～22g的清洁级ICR(Institute of Cancer Researcch,USA)小鼠(Mus musculus),随机分为5个处理组:空白对照组、复方提取液组、黄柏提取液组、白头翁提取液组和白头翁:黄柏(1:1)提取液组。每天按照4g生药/kg体重剂量定时灌胃,空白对照组给予等剂量的蒸馏水。第10天每只小鼠按0.02mL/g体重剂量腹腔注射108cfu/mL大肠杆菌(Escherichia coli)菌液,建立小鼠细菌性致腹泻病理模型,观察各提取液对小鼠的腹泻的影响,第13天收集粪样,变性梯度凝胶电泳(DGGE)研究肠道微生物区系的变化。结果显示,造模当天对照组的体重变化最为明显,腹泻最为严重,与其他各给药组相比均差异显著(P<0.05);4个中草药提取液添加组小鼠的腹泻次数比对照组分别降低了51%、26%、33%和35%,其中复方组抑制小鼠的腹泻效果最为显著(P<0.05);小鼠粪便微生物DGGE图谱分析显示,复方组的条带数和多样性指数均显著高于其他处理组(P<0.05)。研究结果表明,中草药添加剂能够显著地抑制小鼠腹泻,有效地改善肠道微生物菌群结构,其中复方组的效果最优。Fifty ICR（Institute of Cancer Researcch,USA） rats（Mus musculus） with 18～22 g body weight were randomly divided into 5 groups（control,compound Chinese herbal medicine,Pulsatilla,Phellodendron and Pulsatilla：Phellodendron（1：1） group） to investigate the effects of Chinese herb additives on diarrhea inhibition and intestinal bacteria.Rats were given extract by intragastric administration according to 4 g crude drug/kg body weight at the same time everyday,the controls were given distilled water with the same dose.Each rat was given Escherichia coli bacteria according to 0.02 mL/g with 108 cfu /mL by intraperitoneal injection at the 10th day to establish the model of diarrhea,and observe the effect of extract to diarrhea.The dejecta was collected at the 13th day for DGGE analysis.Results showed that,the changes in body weight of the controls were the most obvious at the 10th day,diarrhea was the most serious and significantly different with the other groups（P〈0.05）;The diarrhea frequency of four treatment groups were reduced 51%,26%,33% and 35%,respectively compared with the control group.The diarrhea inhibition of compound Chinese herbal medicine group was significantly higher（P〈0.05） than that of other treatments;The faecal DGGE profiles showed that,for compound Chinese herbal medicine group,thenumber of DGGE bands and diversity index of compound group were more than that of the other treatment groups with significant difference（P〈0.05）.In conclusion,Chinese herb medicine feed additives can significantly reduce diarrhea frequency,optimize the structure of intestinal microbial community in mice,and the best effects were showed in group of compound Chinese herbal medicine.

关 键 词：中草药添加剂 采食量 肠道微生物区系 腹泻率 小鼠 

分 类 号：S963.73[农业科学—水产养殖]