大白猪二脂酰甘油酰基转移酶1和2基因(DGAT1和DGAT2)发育性表达特点及其与背膘厚的关联分析  

Developmental Expression Characteristics of Acyl CoA:Diacylglycerol Acyltransferase Genes 1 and 2(DGAT1 and DGAT2) and Their Association Analysis with Backfat Thickness in Large White Pig

作　　者：王国富[1,2] 高树新[1,2] 张立凡[1] 蔡兆伟[1] 徐宁迎[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]内蒙古民族大学动物科技学院,通辽028000

出　　处：《农业生物技术学报》2011年第4期705-710,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30972078);国家高技术研究发展计划(No.2007AA10Z158)共同资助

摘　　要：二脂酰甘油基转移酶(acry1 CoA:diacylglycerol acyltransferase,DGAT)在细胞甘油脂类的代谢中起着重要的中心作用。本研究应用荧光定量PCR技术,研究了在60、90和120日龄,二脂酰甘油酰基转移酶1和2(DGAT1和DGAT2)基因在大白猪肝脏、皮下脂肪和眼肉中的表达特点,并将组织表达谱与肩部、6～7肋处、胸腰椎接合处和腰荐接合处4个部位的背膘厚进行了关联分析。结果表明,大白猪DGAT1在不同日龄的表达差异均不显著(P>0.05),但DGAT2在60和90日龄的表达差异极显著(P<0.01)。对不同组织DGAT1和DGAT2的表达量分析,DGAT1表达量在肝脏中最高,皮脂次之;DGAT2表达量在皮脂中最高,肝脏次之;眼肉的DGAT1和DGAT2表达量最低。关联分析结果表明,在肝脏中DGAT1表达量与第6～7肋背膘厚(r=0.71,P<0.01)和胸腰椎接合处背膘厚(r=0.62,P<0.01)呈极显著的正相关,在皮脂中DGAT2表达量与前背膘厚呈显著的负相关(r=-0.46,P<0.05)、与第6～7肋背膘厚呈显著的正相关(r=0.49,P<0.05)。研究结果提示,DGAT2基因在2个日龄的表达以及DGAT1和DGAT2基因在3个组织中的相对表达水平均存在差异,并且与部分背膘厚相关显著,可考虑将DGAT1和DGAT2基因作为大白猪背膘厚选育的候选基因。The acyl CoA：diacylglycerpl acyltransferase（DGAT） plays an important central role in the metabolism of cellular diacylglycerol.This study was conducted to investigate the DGAT genes（DGAT1 and DGAT2） expression characteristics during 60,90 and 120 days in 3 different tissues,liver（L）,subcutaneous fat（SF） and rib eye muscle（RE）,by Real-time PCR.The association between tissue expression profile and backfat of shoulder,backfat of 6～7 ribs,backfat of the last rib and backfat of gluteus medius was analyzed.The results showed there were no any difference（P〈0.05） expression of DGAT1 in different days,whereas significant differences（P〈0.05） in DGAT2 mRNA expression between 60 and 90 days in the different tissues of the Large Whites.The analysis of DGAT1 and DGAT2 expression in different tissues indicated that the highest levels of DGAT1 was expressed in the L,followed in SF,the highest levels of DGAT2 was expressed in the SF,followed in L,and the least in ovary of the RE.The result of association analysis showed there were the significant positive correlation between expression of DGAT1 and BF of 6～7 ribs（r = 0.71,P〈0.01） and backfat of the last rib（r = 0.62,P〈0.01） in the L,and the significant negative correlation（r =-0.46,P〈0.05） between expression of DGAT2 and BF of shoulder,and the significant positive correlation（r = 0.49,P〈0.05） between expression of DGAT2 and BF of 6～7 ribs in SF.The results suggested that the expression of DGAT2 gene was different levels in two days,and DGAT1 and DGAT2 were expressed in three tissues with different levels.The expression of DGAT1 and DGAT2 genes were significant correlation with part BF.DGAT1 and DGAT2 can be considered as candidate genes of BF in Large Whites＇ breeding.

关 键 词：大白猪 二酰基甘油酰基转移酶 荧光定量PCR 组织表达谱 背膘厚 

分 类 号：S828.2[农业科学—畜牧学]