肽质量指纹图谱鉴别诊断IgA肾病和非IgA肾病的可行性分析  

Feasibility of peptide mass fingerprinting for differential diagnosis of IgA and non-IgA nephropathy

作　　者：高静[1] 王涌[2] 温新宇[1] 鲁鸿昊[1] 董振南[1] 田亚平[1] 

机构地区：[1]解放军总医院生化科,北京100853 [2]解放军总医院肾脏病科,北京100853

出　　处：《南方医科大学学报》2011年第8期1309-1313,共5页Journal of Southern Medical University

基　　金：国家科技支撑计划(2009BAI86B05)~~

摘　　要：目的探索肽质量指纹图谱(PMF)在IgA肾病和非IgA肾病的分类中是否可行。方法采用基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF MS)分析IgA肾病和非IgA肾病患者的血清肽质量指纹图谱。结果 IgA肾病和非IgA肾病的血清肽质量指纹图谱具有9个差异多肽;其中,最显著的两个多肽峰是4476.46和1968.10,ROC曲线下面积分别为86.18%和79.77%;主因素分析(PCA)表明前8个因素(差异峰)的累积解释变异量超过95%,说明该模型的鉴别诊断能力较MA好L。DI比-T对OF蛋质白谱质技数术据在库Ig,A多肾肽病53和3非8.0I8gA为肾粘病蛋的白分4类同中工,型具的有片可段行;性而,此多技肽术20在82系.7统7为疾α病1-的Ⅱ亚型类胶分原类同中工具型有的广片阔段的。前景结。Objective To investigate the feasibility of peptide mass fingerprinting for non-invasive differential diagnosis of IgA nephropathy（IgAN） from the non-IgA nephropathy（IgAN）.Methods According to the results of renal biopsy,56 patients were divided into IgAN group（n=28） and non-IgAN group（n=28）,and peptide mass fingerprints were acquired from these patients using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS）.Results Nine different peptides were identified between IgAN and non-IgAN.The two most distinctive differentially expressed peptides,with peptide peak values of 4476.46 and 1968.10,showed area under curve values of 86.18% and 79.77%.Principal component analysis demonstrated that the accumulated explained variance of the first 8 differential peptides reached 95%,suggesting the feasibility of differential diagnosis of IgAN from non-IgAN.Comparison with the Matrix protein database identified the peptide with a relative molecular mass of 5338.08 as a fragment of mucin 4 inform and the 2082.77 peptide as fragment of α1-II type collagen inform.Conclusion MALDI-TOF MS is feasible for differential diagnosis of IgAN and non-IgAN and also has great potentials in the classification of the subtypes of other systemic diseases.

关 键 词：IGA肾病 非IgA肾病 肽质量指纹图谱 基质辅助激光解吸电离飞行时间质谱 

分 类 号：Q51-3[生物学—生物化学]