半边旗提取物5F对人胃癌细胞凋亡的影响及其机制  

Effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on human gastric cancer cells and its mechanism

作　　者：陈建发 陈引香 李萍 傅明 吕应年[2] 李立[2] 

机构地区：[1]解放军第422中心医院普外科,广东湛江524009 [2]广东医学院广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《南方医科大学学报》2011年第8期1345-1348,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(3987099)~~

摘　　要：目的探讨草药半边旗提取物Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F)对人胃癌SGC7901细胞凋亡的影响及其机制。方法通过生存及细胞凋亡分析检测5F对SGC7901增殖的抑制效果。同时,采用免疫印迹法评价5F对SGC7901细胞Bcl-2及Bax表达的影响。结果 MTT分析证实了5F呈剂量依赖、时间依赖方式抑制SGC7901增殖。Annexin V-EGFP染色及caspase-3活化则确认了5F诱导的细胞凋亡。并且,该凋亡伴随着Bcl-2表达的降低、Bax表达的提高。结论 5F通过激活细胞凋亡线粒体途径诱导SGC7901细胞凋亡。Objective To investigate the apoptosis-inducing effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid（5F）,a compound isolated from Pteris semipinnata L（PsL）,on human gastric cancer SGC7901 cells and explore its mechanism.Methods The inhibitory effect of 5F on SGC7901 cells was observed by MTT assay and flow cytometry,and the changes of the expression of Bcl-2 and Bax in SGC7901 cells following 5F exposure were evaluated by Western blot analysis.Results 5F inhibited the proliferation of SGC7901 cells in a concentration-and time-dependent manner,and the cell apoptosis induced by 5F was confirmed by Annexin V-EGFP staining and caspase-3 activation assay.The cell apoptosis induced by 5F was associated with decreased Bcl-2 and increased Bax expressions.Conclusion 5F exposure induces apoptosis in SGC7901 cells by activating mitochondrial apoptotic pathways.

关 键 词：半边旗 胃癌 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]