硫化氢对肝硬化胶原蛋白生成的影响  

Hydrogen sulfide regulates collagen formation of the liver cirrhosis WANG Xin-guo

作　　者：王新国[1] 袁建国[2] 张丽旦[1] 万虎[1] 刘雁[1] 吕铁锋[1] 

机构地区：[1]浙江中医药大学附属第六医院感染科,杭州310014 [2]山东省聊城市人民医院感染科,252000

出　　处：《国际流行病学传染病学杂志》2011年第4期235-237,共3页International Journal of Epidemiology and Infectious Disease

摘　　要：目的观察硫化氢（H2s）对肝硬化胶原蛋白形成的影响，探明其在肝硬化形成过程中的作用。方法采用四氯化碳（CCl4）复合因素法建立肝硬化大鼠模型。选取Wistar大鼠20只，按数字表法随机分成正常对照组6只、肝硬化遭模组（造模组）7只和肝硬化造模并加硫化氢钠（NaHS）组（观察组）7只，对观察组进行腹腔注射NaHS1．4gmol／kg，2次／d。4周后，取造模大鼠肝组织行胱硫醚．弘裂解酶（csE）w蕊把m印迹检测和羟脯氨酸测定及H2s检测，分析屿s与羟脯氨酸的相关性。结果经过4周造模，造模组和观察组均有1只大鼠死亡。肝脏病理表现为造模组肝小叶破坏，假小叶形成，而观察组肝硬化程度较造模组轻。与正常对理相比，造模组肝硬化形成后，肝脏内源性H2s含量明显减少，两组的H2S含量分别为（344．544-33．19）和（166．11±50．54）tmaol／L（t=4．366，P〈0．01），肝脏羟脯氨酸含量明显升高，分别为（0．254-0．12）和（2．111-0．86）mg／s（t=5,018，P〈0．01）；与观察组比较，造模组肝脏H2s含量明显增加，分别为（306．974-36．08）和（166．114-50．54）tmaol／L（t=4．297，P〈0．01）；羟脯氨酸明显减少，分别为（1．034-O．41）和（2．114-0．86）mg／g（t=4．874，P〈0．01）。肝硬化过程中CSE含量逐渐降低，但观察组肝脏CASE表达却明显增加，表达水平与正常对照组相似。结论HeS在肝硬化形成过程中，能抑制肝脏胶原蛋白的合成，具有负向调整肝硬化进展的作用。Objective To observe the effect of the hydrogen sulfide on collagen formation in liver cirrhosis and to illustrate its role in the formation of cin＇hosis of the liver. Methods The rat models of liver cirrhosis were induced by compound factors mainly CC14. Twenty wistar rats were divided into normal control group with 6 rats, liver cirrhosis group （modeled group） with 7 rats, liver cirrhosis ＋ NariS group （survey group）with 7 rats, the survey group were injected Naris intraperitoneaUy with 1.4 tunol/kg twice daily and were modeled for 4 weeks. Liver tissue was for the cystathionine- y-lyase（CSE） detection by Western blot and hydroxypreline and hydrogen sulfide determination. The relations between hy- droxyproline and H2S were analysed. Results After 4 weeks of modeling, modeled group and survey group all had one rat dead respectively. The hepatic lobules damaged and pseudlobes formatted in modeled group, while these alterations were slighter in survey group. After the formation of liver cirrhosis, the hydrogen sulfide in control group was significantly reduced significantly compared to modeled group, there were（344.54 ± 33.19） and（ 166.11 ± 50.54 ） panol/L respectively （ t = 4.366, P 〈 0.01） ; and liver hydroxyproline content increased, there were （0.25 ± 0.12） and （2.11 ± 0.86） mg/g respectively（t = 5.018, P 〈 0.01）. Compared to the survey group, the H2S content in modeled group were much higher. There were （306.97 ~ 36.08） and （ 166.11 ± 50.54 ） tanol/L （ t = 4.297 P 〈 0.01 ） respectively. The hydrox- yproline in these two O-oups declined obviously, they were （ 1.03 ± 0.41 ） and （ 2.11 ± 0.86 ） mg/g respectively （ t = 4.874 P 〈 0.01）. The expression of CSE in modeled group decreased gradually, however, the expression of CASE increased sig- nificantly in survey group, the increased level was the same as the normal control group. Condusiom Hydrogen sulfide can negatively regulates the collagen synthesis in the liver and inhibits the

关 键 词：硫化氢 肝硬化 胶原蛋白 

分 类 号：R575.2[医药卫生—消化系统]