应用山梨糖—伊红美蓝培养基分离肠出血性大肠埃希菌O157∶H7的探讨  

The study on SEMB medium for separation of Enterohemorrhagic Escherichia Coli O157:H7

作　　者：刘波[1] 王立霞[1] 吴决[1] 

机构地区：[1]吉林医学院微生物免疫教研室

出　　处：《吉林医学院学报》1999年第2期14-15,共2页

摘　　要：目的：探讨应用山梨糖－伊红美蓝培养基分离肠出血性大肠埃面菌Ｏ１５７：Ｈ７的新途径。方法：用标准野生株肠出血性大肠埃镣菌８８２３６４，以山梨醇－麦康凯培养基（ＳＭＡＣ）作对照，进行细菌的分离培养及菌落计数。结果：肠出血性大肠希菌Ｏ１５７：Ｈ７在ＳＥＭＢ培养基上生长较在ＳＭＡＣ培养基上快。在菌落计数方面二者无显著差异。Objective:To study SEMB medium for separation of Enterohemarrhagic Escherichia Coli O_(157):H_7,Methods:Using SEMB agar,we separated and counted colonies of standard wil strain E.Coli 882364,as compared with SMAC agar.Result:The growth of E.Coli O_(157):H_7 was quicker in SEMB medium than that in SMAC medium.There was no obvious difference on colonies counted.Condusion:We provide a new means to separate E.Coli O_(157):H_7 with SEMB,medium. SEMB medium culture is a rapid,inexpensive,and reliable means of separating E.Coli O_(157).We recommend routine use of SEMB medium.

关 键 词：肠出血 大肠埃希菌 培养基 

分 类 号：R378.2[医药卫生—病原生物学]