丰加霉素对黄瓜立枯丝核菌的拮抗作用  

Antifungal Activity of Toyocamycin on Rhizoctonia solani Kühn

作　　者：于冰[1] 申屠旭萍[1] 俞晓平[1] 

机构地区：[1]浙江省生物计量及检验检疫技术重点实验室/中国计量学院生命科学学院,杭州310018

出　　处：《中国生物防治学报》2011年第3期373-377,共5页Chinese Journal of Biological Control

基　　金：浙江省自然科学基金项目(Y3080238);浙江省公益技术研究项目(2010C32063);杭州市科技计划项目(20101032B72)

摘　　要：采用菌丝生长速率法和盆栽试验法,研究了淀粉酶产色链霉菌Streptomyces diastatochromogenes胞外代谢产物丰加霉素(toyocamycin)对黄瓜立枯丝核菌Rhizoctonia solani的拮抗作用。结果表明:丰加霉素对立枯丝核菌菌丝生长和菌核形成有明显的抑制作用,抑制菌丝生长的EC50值为1.67mg.L-1,浓度为2.20mg.L-1时能完全抑制菌核形成。盆栽试验结果表明,丰加霉素对黄瓜苗立枯病有明显的保护和治疗作用。喷施19.54mg.L-1丰加霉素14d后,对黄瓜苗立枯病的治疗效果为71.91%,显著高于对照霉灵1000倍液的治疗效果(35.83%);喷施19.54mg.L-1丰加霉素14d后,预防效果为51.06%,与对照无显著差异。施药后21d和14d的治疗效果和保护效果均相当;同时19.54mg.L-1和9.77mg.L-1的丰加霉素对黄瓜立枯病的作用效果相当。Effects of toyocamycin against Rhizoctonia solani were tested by mycelial growth rate and pot experiment.Results indicated that toyocamycin strongly inhibited mycelial growth and sclerotium formation of R.solani,with EC50 against mycelial growth at 1.67 mg·L-1.The sclerotium formation of R.solani was entirely inhibited by toyocamycin at 2.20 mg·L-1.After treatment for 14 d,the therapeutic effect of toyocamycin at 19.54 mg·L-1 against R.solani in potted cucumber seedling was 71.91%,significantly higher than that of 1000-fold diluted hymexazol,while the protective effect was 51.06%,without significant difference from that of 1000-fold diluted hymexazol.The protective and therapeutic effects after treatment with 19.54 mg·L-1 and 9.77 mg·L-1 for 14 d and 21 d showed no significant difference.

关 键 词：淀粉酶产色链霉菌 丰加霉素 立枯丝核菌 生防作用 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治] S436.421.19[农业科学—植物保护]