复方滇丹参注射液对大鼠心肌缺血再灌注损伤的保护作用  

Protection Function of Compounded Sanqi and Diandanshen Injection on Myocardium Ischemia/Reperfusion Injury in Rats

作　　者：苏彦宏[1] 段为钢[2] 李惠兰[2] 

机构地区：[1]昆明医学院第一附属医院心内科,云南昆明650032 [2]昆明医学院药理教研室,云南昆明650031

出　　处：《中国血液流变学杂志》2011年第2期231-233,258,共4页Chinese Journal of Hemorheology

基　　金：云南省科委基础研究基金（98c067m）和云南省教育厅应用基础研究基金（03y498c）赞助

摘　　要：目的与复方丹参注射液（丹参十三七）比较，探讨复方滇丹参（滇丹参十三七）对大鼠心肌缺血再灌注损伤的保护作用。方法采用在体和离体缺血心肌再灌注模型，检测血清和灌流液中MDA（丙二醛）、LDH（乳酸脱氢酶）、CK（肌酸激酶）、SOD（超氧化物歧化酶）、GSH-Px（谷胱甘肽过氧化物酶）含量。结果复方滇丹参注射液能显著降低在体和离体缺血再灌注后血清和灌注液中MDA、cK和LDH水平，升高sOD和GsH-Px，作用相似于复方丹参，并能减少心肌梗死面积。结论复方滇丹参注射液有较好的抗心肌缺血再灌注损伤作用，其机制可能与抗氧自由基损伤有关。Objective To find out the cardio-protection induced by the compounded injections made from SY（Diandanshen,S. yunnanesis C. H. Wright） ＋ PN（Sanqi,Panax notoginseng（Burk.） F.H. Chen） during ischemia/reperfusion in rats,compared with injection made from SM（Danshen,Salvia miltiorrhiza Bunge）＋ PN. Methods Ischemia/reperfusion models in vivo and in vitro were employed.Maleic dialdehyde（MDA）,lactate dehydrogenase（LDH）,creatine kinase（CK）,superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in serum or in perfusion fluid after reperfusion were determined. Results Similar to injection of SM ＋ PN, injection of SY＋PN was able to decrease MDA,CK, LDH,and increase SOD,GSH-Px,both in vivo and in vitro.Also similar to injection of SM ＋ PN,injection of SY＋PN was able to decrease ventricle ischemia area in vivo after reperfusion. Conclusion The compounded injection from SY＋PN employs cardioprotection against ischemia/reperfusion injury.The effect is similar to the injection from SM ＋PN.The mechanisms of which may be associated with deactivating oxygen flee radicals.

关 键 词：心肌缺血再灌注损伤 保护作用 复方滇丹参 

分 类 号：R542.2[医药卫生—心血管疾病]