不同亚型穿心莲内酯抗血小板聚集作用的初步研究  

The Study of Antagonistic Effects of Different Andrographolide Subtypes on ADP-induced Platelet Aggregation

作　　者：占葆娥[1] 王晓忠[1] 蒋尊成 曾兰英[1] 索娟 

机构地区：[1]深圳市中医院检验科 [2]药剂科,广东深圳518033

出　　处：《中国血液流变学杂志》2011年第2期326-328,共3页Chinese Journal of Hemorheology

基　　金：广东省建设中医药强省项目（2010307）

摘　　要：目的研究穿心莲内酯、去氧穿心莲内酯、新穿心莲内酯三种不同亚型的穿心莲提取物对二磷酸腺苷（ADP）诱导的血小板聚集的拮抗作用。方法采用醇提取法从穿心莲中提取不同类型的内酯，将其分别加入富含血小板的血浆中，然后用多功能血小板聚集仪测定ADP雳导的血小板聚集作用。结果三种内酯成分均具有抗ADP诱导的血小板凝集作用，以穿心莲内酯为最强，且具有剂量依赖性。结论穿心莲内酯具有较好的抗ADP诱导的血小板聚集作用，具有较好的临床应用前景。Objective To investigate the antagonistic effects of three alcoholic extracts of andrographis paniculata（andrographolide,deoxyandrographolide and neoandrographotide） on ADP-induced platelet aggregation. Methods Three different andrographolide subtypes were extracted from andrographis paniculata using alcoholic extracted method,then added into the human platelet rich plasma,and the ADP-induced platelet aggregation was measured by using multiftmetional platelet aggregometer. Results All three andrographolide subtypes could inhibit the ADP-induced platelet aggregation in vitro,but the andrographolide is the most effective,and this inhibit effect is in a dose-dependent manner.Conclusion Andrographolide can antagonize the ADP-induced platelet aggregation very well in vitro,it has a bright prospect in the clinic use.

关 键 词：穿心莲内酯 血小板 亚型 

分 类 号：R446.11[医药卫生—诊断学]