应用EP7-A2方案评价循环酶法同型半胱氨酸试剂对其他生化检测项目的干扰  

Application EP7-A2 Scheme Evaluation Circulation Enzymatic Homocysteine Reagent for Interference with Other Items on Board Same Analyzer

作　　者：熊声贺[1] 王琴琴[1] 黄新城[1] 

机构地区：[1]南昌大学第三附属医院,江西南昌330008

出　　处：《实用中西医结合临床》2011年第4期67-68,共2页Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的：研究循环酶法同型半胱氨酸检测试剂对尿酸等项目检测的干扰。方法：用CLSIEP7-A2方案,评估循环酶法同型半胱氨酸试剂在生化分析仪中,因携带交叉污染对常用项目检测的干扰。结果：检测结果显示,在模拟污染率0.1%-0.5%范围内,ALT、AST从0.1%污染率开始,结果均超出（X-1.96s）范围;GSP从0.2%污染率开始,结果均超出（X＋1.96s）范围;UA从0.3%污染率开始,结果均超过（X-1.96s）范围;Mg从0.3%污染率开始,结果均超出（X＋1.96s）范围;其他15项目检测结果均在（X±1.96s）范围内。结论：生化分析仪携带污染率在0.5%的允许范围内,循环酶法同型半胱氨酸检测试剂可能对同一检测系统中,糖化血清蛋白（GSP）等5项检测造成干扰,而对总胆红素等其他15项检测不造成干扰。Objective：To study uric acid et.detecting interferenced of the circulation enzymatic homocysteine reagent.Methods：Using CLSI EP7-A2 scheme,evaluate circular enzymatic homocysteine reagent in biochemistry analyzer,because carry cross contamination of interference to commonly used analysis items detection.Results：The simulation tests showed that the rate of pollution within 0.1%~0.5%,from 0.1% rate of pollution,the ALT and AST test results exceed（X-1.96s） limit;from 0.2% rate of pollution,the GSP test results exceed（X＋1.96s） limit;from 0.3% rate of pollution,the uric acid test results exceed（X-1.96s） limit;from 0.3% rate of pollution,the Mg test results exceed（X＋1.96s） limit.Other 15 projects are testing results within（X±1.96s）.Conclusion：Biochemical analyzer to carry the rate of pollution in the range of allowable 0.5%,circulation enzymatic homocysteine reagent in the same detection system,saccharification serum protein（GSP） and five test cause interference,but to total bilirubin and other 15 test don＇t cause interference.

关 键 词：同型半胱氨酸 糖化血清蛋白 生化分析仪 携带交叉污染 

分 类 号：R446.1[医药卫生—诊断学]