Caspase-3表达强度评价齿科合金生物相容性的实验研究  

The Evaluation of Dental Alloys Biocompatibility by Detecting Expression of Caspase-3

作　　者：李秀梅[1] 战德松[2] 

机构地区：[1]辽宁医学院口腔医学院,辽宁锦州121001 [2]中国医科大学口腔医学院,辽宁沈阳110002

出　　处：《中国医学工程》2011年第7期70-71,73,共3页China Medical Engineering

摘　　要：目的利用凋亡相关因子caspase-3的表达强度分析其与齿科合金生物相容性的相关性。方法以银钯合金、镍铬合金、钴铬合金、自制2号合金的浸提液(每组样本量均为8个)体外培养小鼠成纤维细胞(L929),以含10%胎牛血清的RPMI 1640培养基作为阴性对照组。用5种液体分别培养L929细胞48h后,收集细胞,提取蛋白,染色后测定OD值,计算caspase-3活化度。结果各实验组Caspase-3活性比较有显著性差异(P<0.01)。Caspase-3活化度大小,由高到低:钴铬合金组>2号合金组>银钯合金组>镍铬合金组。结论各实验组细胞凋亡程度由高至低依次为:钴铬合金与2号合金>银钯合金>镍铬合金。凋亡相关因子Caspase-3在时间梯度的辅助下与微核试验、鼠伤寒沙门氏菌回复突变试验、MTT法评价2号合金生物相容性结果一致。【Objective】 To analyze the relationship between dental alloys biocompatibility and apoptosis correlation factor caspase-3.【Methods】 Cultivated L929 cells by Ag-Pd alloys leaching liquor,Ni-Cr alloys leaching liquor,Co-Cr alloys leaching liquor,self-made 2# alloys leaching liquor（each group sample capacity was 8） as control.Cultivated L929 cells by RPMI 1640 cell medium containing 10% fetal calf serum as negative control.Collected cells,extracted proteinum,dyed,detected OD,calculated the activity of caspase-3 after 48h.【Results】 The cell quantity of each group were difference,there were lots of apoptosis cells in each group,and orange cells could be seen in Ni-Cr alloys group after 48h.The activity of caspase-3 in each group was obviously different（P0.01）.From high to low was： Co-Cr alloys group 2# alloys group Ag-Pd alloys group Ni-Cr alloys group.【Conclusion】 Degree of apoptosis in each group from high to low was： Co-Cr alloys group and 2#alloys group Ag-Pd alloys group Ni-Cr alloys group.Caspase-3 the correlation factor of apoptosis which assisted by time gradient in evaluating 2# alloys biocompatibility was the same as micronucleus tests,.Ames test and MTT.

关 键 词：生物相容性 细胞凋亡 CASPASE-3 分光光度法 

分 类 号：R276.8[医药卫生—中医五官科学]