荷花品种过氧化物同工酶研究  

Study on the peroxidase isozyme in lotus cultivars

作　　者：赵可新[1] 钱萍[2] 孙德荣 唐宇力 朱诚[4] 胡家恕 童富淡 

机构地区：[1]杭州市园文局,浙江杭州310006 [2]杭州市园文局灵隐管理处,浙江杭州310007 [3]杭州市植物园,浙江杭州310013 [4]浙江大学生物科学系,浙江杭州310029

出　　处：《浙江农业科学》1999年第6期288-291,共4页Journal of Zhejiang Agricultural Sciences

基　　金：杭州市园文局科技基金

摘　　要：用聚丙烯酰胺等电聚焦系统测定了２６ 个荷花品种过氧化物酶同工酶，共出现酶带有１６条， 等电点在ｐＨ４０～６０ 之间， 荷花品种的特征谱带有４ 条， 即ｐＨ 为４３２， ４７８， ４８８， ５０４。根据荷花品种中各酶带出现的频率 （Ｆ）， 计算各品种谱带的相同值 （ＳＩ）， 进行分析。The peroxidase isozyme patterns in leaves of 26 lotus cultivars were analysed via polyacrylamide isoelectrofocusing technique. Results of the analysis indicated that peroxidase could be divided into 16 isozyme bands while PI being in the range of pH 4 0-6 0, and that there were 4 characteristc isozyme bands in common, namely the PI was 4 32, 4 78, 4 88 and 5 04, respectively. The SI values for each cultivar′s bands were calculated, according to the frequency of each isozyme band of corresponding cultivars. The evolution of some cultivars and methods used in this research were discussed.

关 键 词：荷花 电泳酶谱分析 过氧化物同工酶 品种 

分 类 号：S682.320.1[农业科学—观赏园艺] Q554.6[农业科学—园艺学]