杜氏盐藻S腺苷高半胱氨酸水解酶基因的克隆及功能分析  

Cloning and functional analysis of S-Adenosyl homocysteine hydrolase gene of Dunaliella salina

作　　者：阎赟梦[1] 李庆华[1] 李杰[1] 柴丹丹[1] 薛乐勋[1] 

机构地区：[1]郑州大学生物工程系细胞生物学研究室,郑州450001

出　　处：《郑州大学学报（医学版）》2011年第4期517-520,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目3070014;科技部国际科技合作基金资助项目2007DFA01240

摘　　要：目的:探讨杜氏盐藻S腺苷高半胱氨酸水解酶(SAHase)基因在鞭毛再生中的作用。方法:通过设计简并引物及RACE法克隆了杜氏盐藻SAHase基因全长,使用pH休克法对杜氏盐藻进行鞭毛去除及再生,通过实时荧光定量PCR研究在转录水平上SAHase基因的变化。结果:所克隆的杜氏盐藻SAHase基因cDNA全长1926bp,包含ORF1458bp、3’UTR61bp和5’UTR407bp。实时荧光定量PCR结果显示,在鞭毛再生的过程中,SAHasemRNA转录量升高,其水平在3h时达到了未处理组的3倍左右。结论:杜氏盐藻的SAHase基因与鞭毛再生有关。Aim：To investigate the function of the SAHase gene in flagellar regeneration of Dunaliella salina（D.salina）.Methods：A pair of degenerate primers was designed to obtain a fragment of SAHase cDNA,and the RACE method was used to amplify the full length of the SAHase cDNA.The transcriptional level of the SAHase gene from D.salina was monitored by real-time fluorescence quantitative PCR during flagellar regeneration.Results：The results of PCR and RACE showed that the full length of the SAHase cDNA cloned in this study was 1 926 bp,consisting of ORF 1 458 bp,3＇ UTR 61 bp and 5＇ UTR 407 bp.The result of real-time fluorescence quantitative PCR indicated that abundance of the SAHase mRNA was induced during the process of the flagellar regeneration,reaching to about 3-fold higher than that without the treatment of pH shock.Conclusion：The SAHase gene is related to regeneration of the flagella of D.salina.

关 键 词：杜氏盐藻 S腺苷高半胱氨酸水解酶 鞭毛 

分 类 号：Q781[生物学—分子生物学]