黄瓜CsCCD7基因的克隆及表达研究  

Cloning of CsCCD7 and Its Expression in Cucumis sativus L.

作　　者：徐庆华[1] 胡宝忠[2] 李凤兰[2] 金峰[2] 王多佳[2] 

机构地区：[1]东北农业大学,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《热带亚热带植物学报》2011年第4期365-373,共9页Journal of Tropical and Subtropical Botany

基　　金：国家"863"计划项目(2007AA10Z177);高等学校博士点专项科研基金(200802240008)资助

摘　　要：使用RACE技术克隆黄瓜(Cucumis sativus L.)腋芽生长抑制基因并进行生物信息学和半定量RT-PCR分析。结果表明:从黄瓜腋芽中成功克隆了拟南芥(Arabidopsis thaliana)AtCCD7/MAX3同源基因,命名为CsCCD7(GenBank登录号:HQ005419);CsCCD7基因序列含有1665 bp的开放阅读框(ORF),编码554个氨基酸;编码的蛋白质命名为CsCCD7,隶属于CCD蛋白家族成员,蛋白质的二级结构和三级结构预测显示其富含β折叠和β转角以及无规卷曲,是不稳定蛋白。CsCCD7在根中的表达量最高,在多分枝、矮化黄瓜D0462中的表达量最低,这说明CsCCD7蛋白可能参与调控植物分枝信号的转导及分枝相关基因的表达调控。The cucumber（Cucumis sativusL.） inhibiting axillary bud growth gene,CsCCD7,had been cloned（GenBank accession number： HQ005419） using by RACE,and its bioinformatics and semi-quantitative RT-PCR analysis were studied.The results showed that the CsCCD7 was the homologue gene of AtMAX3/CCD7in Arabidopsis thaliana,which contain ORF with 1665 bp,and code the protein with 554 amino acids,named CsCCD7,belonging to CCD protein family.The secondary and tertiary structure prediction of CsCCD7 showed having rich β sheets,β turns and irregular curls,which indicated it was unstable protein.CsCCD7 expression in roots was the highest,while that in dwarf cucumber ‘D0462’ with lot of branches were the lowest by RT-PCR analysis.It suggested that the CsCCD7 protein could revolve in control branch signal transduction and related gene expression in cucumber.

关 键 词：黄瓜 腋芽生长抑制基因 基因克隆 生物信息学分析 基因表达 

分 类 号：S642.2[农业科学—蔬菜学]