大孔树脂对发酵液中松脂醇二葡萄糖苷的吸脱附性能  

ADSORPTION AND DESORPTION PROPERTIES OF MACROPOROUS RESIN TO PINORESINOL DIGLUCOSIDE IN LIQUID CULTURE

作　　者：刘超[1] 师俊玲[1] 刘拉平[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,杨凌712100

出　　处：《离子交换与吸附》2011年第4期342-352,共11页Ion Exchange and Adsorption

基　　金：陕西省科技攻关项目(2004K03-G5-02)

摘　　要：考察了S-8、AB-8、NKA-II、NKA-9 4种大孔吸附树脂对杜仲内生真菌拟茎点霉属XP-8发酵液中松脂醇二葡萄糖苷(Pinoresinol Diglucoside,PDG)的吸附和脱附性能,筛选出S-8树脂的吸附和脱附性能最好;探讨了S-8树脂在静态吸附条件下对发酵液中PDG的吸附平衡和吸附动力学,考察了温度和pH值对吸附效果的影响;进行了动态吸附实验,确定了最佳吸附和洗脱条件。结果表明,在静态吸附条件下,Langmuir方程可很好地描述PDG在S-8树脂上的吸附平衡,液膜扩散和颗粒内扩散分别是控制吸附初期和后期吸附速率的主要步骤;动态吸附的最佳条件是,上样浓度为0.195mg/mL、上样温度为20℃、pH 9、进样流速1BV/h,溶液处理量20BV;最佳动态洗脱条件是,洗脱液为30%的乙醇水溶液,洗脱液流速1BV/h,用量为6BV。整个动态吸附洗脱过程结束后的PDG得率为89.8%。Four macroporous resins, including of S-8, AB-8, NKA- II and NKA-9, were studied on separation and purification of Pinoresinol Diglucoside （PDG） in the liquid culture of Phomopsis sp., a strain isolated from Eucommia ulmoides Oliv. The experimental results showed that S-8 resin could achieve better adsorption and desorption performance than others. With S-8 resin, equilibrium and kinetics of static adsorption of PDG were studies in detail and the effects of temperature and pH on the adsorption performance were also discussed. The dynamic adsorption and desorption conditions of S-8 resin for PDG were optimized. The results indicated that isothermal sorption process of PDG on S-8 resin could be well described by Langmuir equation, and the adsorption rate of the initial stage and the later stage were controlled by liquid film diffusion and inner-particle diffusion, respectively. The optimum dynamic adsorption conditions for PDG were as follows： PDG concentration, 0.195mg/mL; pH 9; adsorption temperature, 20℃; flow rate, 1BV/h; and saturated adsorption amount of PDG 20BV. The optimum desorption conditions were as follows： eluant, 30% ethanol aqueous solution （v/v）; the amount of elution, 6BV and eluant flowing rate, 1BV/h. In these techniques, high recovery efficiency of PDG as much as 89.8% was obtained.

关 键 词：松脂醇二葡萄糖苷 大孔树脂 拟茎点霉属发酵液 吸附 脱附 

分 类 号：O647.3[理学—物理化学]