两种贵州地方鸡线粒体DNA控制区全序列分析  

Analysis on the mtDNA Control Region of Two Kinds of Guizhou Native Chicken

作　　者：王文涛[1] 陈彬[1] 傅筑荫[2] 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳550025 [2]贵州大学动物科学学院,贵阳550025

出　　处：《湖北农业科学》2011年第15期3114-3115,3119,共3页Hubei Agricultural Sciences

基　　金：贵州省科技厅攻关项目[黔科合NY字(2007)3019]

摘　　要：采用PCR和直接测序的方法测定兴义矮脚鸡和长顺绿壳蛋鸡线粒体DNA控制区全序列,分析比较两者mtDNA D-loop序列3个区的变异。结果表明,兴义矮脚鸡和长顺绿壳蛋鸡线粒体DNA控制区全序列长分别为1 231和1 230 bp,其中兴义矮脚鸡mtDNA控制区的碱基摩尔分数分别为A 26.92%、T33.41%、G 13.39%、C 26.29%,长顺绿壳蛋鸡的分别是A 27.13%、T 33.58%、G 13.20%、C 26.09%。t检验显示两种贵州地方鸡种间线粒体DNA控制区碱基A与C的含量差异显著,说明A与C之间的颠换较多。兴义矮脚鸡和长顺绿壳蛋鸡的遗传距离是0.038 6,根据分子钟计算,二者大约在193万年前分歧进化。PCR and direct sequencing methods were used to analyze the entire mtDNA D-loop in Xingyi Bantam Chicken and Chang shun Blue-shelled Egg Chicken.The D-loop of Xingyi Bantam and Changshun Blue-shelled Egg Chicken was 1 231 and 1 230 nucleotides long,respectively.The nucleotide molar fraction of Xingyi Bantam was A 26.92%,T 33.41%,G 13.39%,and C 26.29%,while the compsition of Changshun Blue-shelled Egg Chicken was A 27.13%,T 33.58%,G 13.20% and C 26.09%.The t-test results showed that the difference of the nucleotide A and C content in the mtDNA control region of the two chicken breeds was significant.The genetic distance between the two breeds was 0.038 6.Calculated by molecular clock,the two breeds diverged and evolved about 1.93 million years ago.

关 键 词：线粒体DNA控制区 兴义矮脚鸡 长顺绿壳蛋鸡 遗传变异 

分 类 号：Q343.3[生物学—遗传学]